TRACE ANESTHETIC & METABOLITE IN CHIRAL & RACEMIC MIXTURES OF SECONDARY ALCOHOLS

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 2-酒精是麻醉剂。 我们一直在研究这些化合物在动物和表达的离子通道上的麻醉作用。 我们在动物和受体中观察到这些化合物的不寻常效价模式，表明可能存在杂质。 虽然这些化合物以约99%的纯度购买，但可能存在挥发性和非常亲脂性的痕量化合物，并产生显著的麻醉作用。 通过气相色谱法观察到几种痕量化合物。 我们需要质谱来确定这些痕量化合物是什么，以评估它们是否具有麻醉剂的作用。 这将允许我们知道所观察到的效力关系是真实的，这将具有机械的意义，还是由杂质引起的人工产物。 此外，动物体内似乎也会产生代谢物，这也需要鉴别，以便校正仲醇对该化合物存在的效价。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. 2-alcohols are anesthetics. We have been studying the anesthetic effects of these compounds in animals and on expressed ion channels. We have observed an unusual potency pattern for these compounds both in animals and on receptors suggestion that an impurity might be present. Although these compounds are purchased in approximately 99% purity, a trace compound that is volatile and very lipophilic may be present and exerting significant anesthetic effects. Several trace compounds are observed by gas chromatography. We need mass spectrometry to determine what these trace compounds are in order to evaluate whether they have actions as anesthetics. This will permit us to know whether the observed potency relations are real, which would have mechanistic significance, or an artifact caused by an impurity. In addition, a metabolite appears to be produced in animals and this too requires identification, in order to correct the potency of the secondary alcohol for the presence of this compound.