GROEL VARIANT

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 以前的研究无法捕获和研究GroEL-GroES复合物的预触发状态并不奇怪，因为野生型GroEL只能短暂地填充这种变构状态（大约100毫秒），对于准备操作来说时间太短。 相比之下，我们修改后的GroEL变体似乎以相当慢的速度通过这种转变。 我们建议对这种新的GroEL变体进行高分辨率的观察，利用NCMI在通过cryo-EM检查GroEL等分子方面取得的技术进步，可能会提供我们目前所知甚少的GroEL-GroES机器的关键变构状态的前所未有的快照。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The inability of previous studies to trap and study a pre-triggered state of the GroEL-GroES complex is not surprising, given that wild-type GroEL populates this allosteric state only transiently (for a hundred millisec or so), much too short a time for ready manipulation. By contrast, our modified GroEL variant appears to move through this transition at a considerably slower rate. We propose that a high-resolution look at this new GroEL variant, exploiting the technical advances the NCMI has made in examining molecules like GroEL by cryo-EM, is likely to provide an unprecedented snap-shot of a critical allosteric state of the GroEL-GroES machine about which we currently know very little.