COPOLYMER-MEMBRANE PROJECT

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 泊洛沙姆是一类表面活性嵌段共聚物，由疏水的聚环氧丙烷(PPO)部分组成，两端覆盖有亲水性的聚环氧乙烷(PEO)部分，已知通过阻止受损细胞内物质的泄漏来帮助密封因疾病或创伤而受损的通透性细胞膜。尽管泊洛沙姆在恢复细胞膜完整性方面很重要，但其潜在的封闭机制还不是很清楚。泊洛沙姆188(p188，分子量=8400g/mol，含80wt%PEO)因其在低毒药物中的广泛应用而成为第一个被测试的膜密封剂家族。此外，p188是一种高效的基因载体，可通过内吞作用实现基因转移。P188和其他泊洛沙姆也被包括在药物输送系统中，通过延长脂质体的循环时间来对其进行空间稳定。用于制药的泊洛沙姆设计的改进依赖于对泊洛沙姆和脂膜之间相互作用的更好理解。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Poloxamer, a family of surface-active block copolymers constituted of a hydrophobic poly (propylene oxide) (PPO) moiety capped with hydrophilic poly (ethylene oxide) (PEO) moieties on the two ends, is known to help seal permeabilized cell membranes that are damaged by disease or trauma via arresting leakage of intracellular materials of the damaged cell. Despite poloxamer's importance in restoring cell membrane integrity, the underlying sealing mechanisms are not well understood. Poloxamer 188 (P188, molecular weight = 8400 g/mol, with 80 wt% PEO) was the first among its family tested to be a membrane sealant due to its wide application in pharmaceuticals with low toxicity. Besides, P188 is an efficient gene carrier with gene transfer accomplished via endocytosis. P188 and other poloxamers have also been included in drug delivery systems to sterically stabilize liposomes by prolonging their circulation time. Improvement on poloxamer design for pharmaceutical use relies on a better understanding of interactions between poloxamer and lipid membranes.