HIGH-RESOLUTION EX VIVO IMAGING OF AN ALZHEIMER'S MOUSE MODEL

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 使用MR 9T磁体进行本研究。MouseBIRN合作小组寻找试验床案例，以生成可搜索的在线图谱，该图谱结合了神经退行性疾病小鼠模型的基因表达和3D MRI图像。在这种情况下，我们想要开发出阿尔茨海默病的新的tet-off应用程序小鼠模型。研究工作已经在进行中，以生成tet-off app小鼠在淀粉样变进展过程中的多个时间点的基因表达谱。与CIVM合作提出的成像将提供全面的、高分辨率的3D形态图像，以补充基因表达分析。我们将专注于评估基因表达的五个时间点中的两个，一个在淀粉样蛋白沉积开始之前，一个在淀粉样蛋白沉积开始之后。其目标是在每个时间点开发5只阿尔茨海默氏症(Tet-Off APP)和5只对照(TTA)动物的平均渲染，不仅可以用作MouseBIRN自动数据分析管道的测试，还可以提供有关特定大脑区域随着疾病进展而发生的体积和/或结构变化的有价值的信息。这项研究的数据将通过MouseBIRN网络公开。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Using MR 9T magnet for this study. The MouseBIRN collaborative group seeks testbed cases to generate a searchable, online atlas that combines gene expression and 3D MRI images of mouse models for neurodegenerative disease. We would like to develop the new tet-off APP mouse model of Alzheimer's disease in this context. Work is already under way to generate gene expression profiles of the tet-off APP mice at multiple points in the progression of amyloidosis. The imaging proposed in collaboration with CIVM would provide comprehensive, high-resolution, 3D morphological images to complement the gene expression analyses. We will focus imaging efforts on two of the five time points assessed for gene expression, one before and one after the onset of amyloid deposition. The goal is to develop an average rendering from 5 Alzheimer's (tet-off APP) and 5 control (tTA) animals at each time point that can be used not only as a test of the MouseBIRN automated data analysis pipeline but also provide valuable information about volumetric and/or structural changes in specific brain areas as the disease progresses. Data from this study will be made publicly available through the MouseBIRN network.