ULTRASONIC STIFFNESS IMAGING OF RAT LIVER

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 在活体大鼠上的项目，BME的超声系统。 我们计划使用我们定制的超声成像系统对肝脏硬度进行成像。对于这项初步研究，我们希望获得回答以下问题的初始数据：1)鉴于存在相当大的心脏和呼吸运动问题，我们能否将大鼠肝脏与我们的系统一起成像。在人身上，我们使用心电触发和屏气，但我们需要在提交拨款之前证明或评估我们将在大鼠模型中遇到的挑战。在拨款中，我们计划建议使用我们的超声成像系统对肝脏硬度进行纵向监测，并希望将我们的硬度图像与组织学肝纤维化分期相关联，在我们发现肝脏脂肪变性/进行性纤维化的大鼠模型中(即CCl4)，我们需要回答的问题是：我们能否在活体动物身上通过呼吸机纵向做到这一点，或者运动问题太繁琐，以至于我们将需要执行单一时间点研究。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Project on live rats, on ultrasound system from BME. We plan to use our customized ultrasonic imaging system to image liver stiffness. For this initial study, we would like to obtain intial data answering the following questions: 1) can we image rat livers with our system, given the considerable cardiac and respiratory motion issues that exist. In people, we use ECG triggering and breath holds, but we need to demonstrate or assess the challenges we will encounter in a rat model prior to submitting our grant. In the grant we plan to propose longintudinal monitoring of liver stiffness with our ultrasound imaging system, and we want to correlate our stiffness images with histologic liver fibrosis stage, in a rat model for hepatic steatosis/progressive fibrosis that we have found is widely used in the liver (i.e. CCL4) The question we need to answer is: can we do this longitudinally in live animals on a ventilator, or are the motion problems too onerous, such that we will need to perform single time point studies.