CORE--DNA MICROARRAY & GENOMICS

微阵列和基因组学资源提供了一套集成的工具和服务，用于 应用基因组学的吞吐量技术。资源对人类基因组计划的重要性通过克隆生成、高通量映射、阵列技术开发以及这些资源在全球的分布来证明，展示了专业知识和成就的跟踪记录。微阵列和基因组资源由两个互补的部门组成，基因组部门通过赠款、合同和按存储容量使用计费收入提供资金，并为应用 人和小鼠BAC克隆用于下游功能分析。它通过机器人技术为大型项目提供自动化基础设施。微阵列部门的资金来自CCSG、RPCI支助和按存储容量使用计费收入，EDNA和基因组克隆，如基因组部门项目中确定的那些，作为阵列和存档资源提供。研究人员可以请求微阵列服务，包括RNA/DNA分离、Cy3和Cy5探针生成、杂交、定制的cDNA和基因组阵列、扫描微阵列载玻片、图像分析、数据标准化以及与Sybase关系数据库的基于网络的接口。除EDNA文库外，还有9个BAC/PAC基因组文库可用于筛选、克隆选择、鉴定、作图和分布 在RPCI内。在上一个项目期间，79%的使用率是由CCSG成员利用同行审查的资金。该战略计划将继续提供获得最先进技术的途径，促进应用基因组学的大规模和/或全球分析。主任与CCSG领导层和成员密切合作，指导、教育和支持基因组工作。

The Microarray & Genomics Resource provides an integrated set of tools and services for high throughput technologies for applied genomics. The importance of the Resource to the Human Genome Program is documented by the contributions of clone generation, high throughput mapping, array technology development, and distribution of these resources worldwide, demonstrating a track record of expertise and accomplishment. The Microarray & Genomics Resource is composed of two complementary divisions, The Genomics division is funded through grants, contracts, and chargeback revenues, and contributes to the application of human and mouse BAC clones for downstream functional analysis. It provides the infrastructure for automation through robotics for large-scale projects. The Microarray division is funded through the CCSG, RPCI support and chargeback revenues, eDNA and genomic clones, such as those identified in the Genomics division projects, are offered as an arrayed and archived resource. Investigators can request microarray services including RNA/DNA isolation, Cy3 and Cy5 probe generation, hybridizations, custom-made cDNA and genomic arrays, scanning of microarrayed slides, image analysis, normalization of data and web-based interface with Sybase relational database. In addition to the eDNA libraries, nine BAC/PAC genomic libraries are available for screening, clone selection, characterization, mapping, and distribution within RPCI. In the last project period, 79% of the utilization was by CCSG members with peer-reviewed funding. The Strategic Plan is to continue to provide access to state of the art technology facilitating large scale and /or global analyses for applied genomics. The Director works closely with the CCSG leadership and members to guide, educate, and support genomic endeavors.