DNA ANALYSIS/AUTO SEQUENCING AND PHOSPHORIMAGING

DNA分析共享资源 Thomas Tedder，博士，主任 杜克综合癌症中心DNA分析共享资源由两个相关设施组成， 磷光成像仪设备和DNA测序设备。该资源服务于跨学科的 来自癌症中心、医学中心和大学的调查小组。这些调查人员来自 基础和临床科学领域都有广泛的兴趣，包括癌症生物学，基础 分子生物学、信号转导、发育机制、细胞对感染的反应和人类 癌症、免疫缺陷、关节炎和心脏病等疾病。磷光成像仪设施允许 研究人员使用放射自显影、荧光和放射自显影技术分析各种DNA和蛋白质样本 化学荧光检测。这项技术使科学家能够轻松地进行准确的信号检测 在数量上超过几个数量级，同时减少曝光时间。DNA测序 设施提供自动DNA测序、DNA片段分析和突变检测 基因结构和功能的分析。这两个设施结合在一起，提供了一种集成的方法 以满足杜克大学社区的DNA分析需求。此外，研究还通过以下方式加快 通过替换较旧、低效且成本高昂的手动操作，显著节省数据生产时间 方法采用最先进的自动化程序。这种资源提供了新的基于荧光的 减少我们对放射性使用的依赖的技术。

DNA ANALYSIS SHARED RESOURCE Thomas Tedder, Ph.D., Director The Duke Comprehensive Cancer Center DNA Analysis Shared Resource consists of two related facilities, the Phosphorimager Facility and the DNA Sequencing Facility. This resource serves an interdisciplinary group of investigators from the Cancer Center, Medical Center and University. These investigators come from both the basic and clinical science areas with a wide range of interests including cancer biology, basic molecular biology, signal transduction, mechanisms of development, cellular response to infection and human diseases such as cancer, immunodeficiency, arthritis and heart disease. The Phosphofimager Facility allows researchers to analyze a wide variety of DNA and protein samples using autoradiography, fluorescence, and chemifluorescence detection. This technology permits scientists to easily perform accurate signal detection quantitatively over several orders of magnitude along with reduced exposure times. The DNA Sequencing Facility provides automated DNA Sequencing, DNA fragment analysis and mutation detection for rapid analysis of gene structure and function. Together, these two facilities combine to offer an integrated approach to serving the DNA analysis needs of the Duke University community. In addition, research is expedited by providing significant time savings in the production of data by replacing older, inefficient, and costly manual methods with state-of-the art, automated procedures. This resource makes available new fluorescence based techniques that reduce our dependence on the use of radioactivity.