Shared Resources-Microarray

微阵列共享资源(MSR)于1999年启动，旨在快速、经济高效地提供 用于癌症研究的微阵列技术。MSR包含一个全功能的斑点微阵列 具有3,548 ff空间并打印cDNA、寡核苷酸和蛋白质玻片微阵列的设施 100级洁净室中最高的质量。点阵服务包括： 一)全基因组和靶向人类和小鼠斑点DNA微阵列和方案的可用性， 2)全面的项目服务，包括标记、杂交、扫描和初级数据分析； 三)为非标准应用程序的专门、调查员定义的印刷项目提供定制阵列服务， 四)市场上可获得的蛋白质微阵列的完整项目服务(例如Clontech Ab500)和 蛋白质的定制打印。调查人员能够使用所提供的任何或所有工具和服务 要求。自成立以来，已有68个YCC成员使用了MSR斑点微阵列服务。这 代表了所有癌症中心成员的22%。在2005财年，19名YCC调查人员占18% 在3550个斑点玻片微阵列中，提供了服务。 包括在MSR中的Affymetrix基因芯片部分成立于2001年，以支持 利用基于Affymetrix寡核苷酸的微阵列进行大规模基因表达研究。团结在一起， 基因芯片和斑点玻璃载玻片微阵列技术为 有可能帮助确定疾病的生化原因的癌症研究正在发展 更好的诊断方法，并改善治疗结果和病人护理。的基因芯片部分 MSR占用了1753平方英尺的新建、定制设计的实验室空间，设备齐全 拥有基因芯片仪器系统和ABI序列检测系统。可用的Affymetrix服务 包括一)表达分析，包括RNA/信使核糖核酸提取、适合于 阵列杂交和数据分析，ii)使用Affymetrix进行SNP分析(10、100和500K分析) 和ABI平台，以及iii)使用ABI 7900序列对微阵列结果进行定量PCR验证 探测系统。MSR的玻片微阵列和Affymetrix基因芯片部分都将参考 根据需要进行实验设计、数据解释、稿件和拨款申请准备，并将 提供标记、杂交和原始数据分析方面的培训。2005年间，25名YCC调查人员 使用Affymetrix基因芯片的服务占所提供的2334项服务的22%。自成立以来， Affymetrix部门已为93名耶鲁大学调查人员和30名其他机构的调查人员提供了6000项服务。

The Microarray Shared Resource (MSR) was initiated in 1999 to rapidly and cost-effectively bring the power of microarray technology to bear on cancer research. The MSR contains a fully-functional spotted microarray facility that has 3,548 ff space and prints cDNA, oligonucleotide, and protein glass slide microarrays of the highest possible quality in class 100 cleanrooms. Spotted array services include: i) availability of whole genome and targeted human and mouse spotted DNA microarrays and protocols, ii) complete project services including labeling, hybridization, scanning and primary data analysis, iii) custom array services for specialized, investigator defined, printing projects for non-standard applications, iv) complete project services for commercially available protein microarrays (e.g. Clontech Ab500) and custom printing of proteins. Investigators are able to use any or all of the tools and services offered as they require. 68 YCC members have utilized MSR spotted microarray services since its inception. This represents 22% of all Cancer Center members. During CY 2005, 19 YCC investigators accounted for 18% of the 3,550 spotted glass slide microarray provided services. The Affymetrix GeneChip section, which is included in the MSR, was established in 2001 to support large-scale gene expression studies utilizing Affymetrix oligonucleotide based microarrays. Together, the GeneChip and spotted glass slide microarray technologies offer comprehensive microarray support for cancer research which has the potential to aid in identification of biochemical causes of the disease, develop better diagnostic methods, and to improve therapeutic outcomes and patient care. The GeneChip section of the MSR occupies 1,753 ft2 of newly constructed, custom-designed laboratory space and is well equipped with GeneChip instrument systems and an ABI Sequence Detection system. Available Affymetrix services include i) expression analysis that includes RNA/mRNA extraction, preparation of target samples suitable for array hybridization, and data analysis, ii) SNP analysis using both Affymetrix (10, 100, and 500 K assays) and ABI platforms, and iii) Quantitative PCR validation of microarray results using the ABI 7900 Sequence Detection system. Both the glass slide microarray and Affymetrix GeneChip sections of the MSR will consult as needed on experimental design, data interpretation, manuscript and grant application preparation, and will provide training for labeling, hybridization and primary data analysis. During CY 2005, 25 YCC investigators using Affymetrix GeneChip services accounted for 22% of the 2,334 provided services. Since inception, the Affymetrix section has provided >6,000 services to >93 Yale investigators and 30 from other institutions.