Animal Care Core

允许研究黏附分子在输血生物学中的功能的动物模型是 这个节目的中心主题。毫无疑问，动物基因工程技术是一种 是近年来生物学最大的进步之一。不幸的是，动物实验并不简单。在……里面 特别是，存在可变性：经过基因改造的动物并不是真正的彼此克隆，因此 他们对治疗的反应可能不同。如果老鼠是混合背景的，情况尤其如此 没有完全回交到特定的遗传背景。为了将可变性降到最低，使用了野生动物 从相同的杂合子配对中进行比较，或者至少从相同的动物中进行比较 殖民地。因此，突变动物和野生动物通常都必须被安置。所以除了变种人 在表1中列出的菌株中，有几个野生型菌株需要保存。获得统计数据 为了得到有意义的结果，每个实验必须至少有6-10只每种基因的动物参与。 动物收容所的成本正变得令人望而却步。这一核心单元是该计划之所以 最初放在一起。能够维持和繁育大量的某一种基因的小鼠 实验目的，一个由三个繁育笼子组成的小鼠群体(维持线)外加 需要额外的养殖和实验笼子(用于扩展)。在我们的经验中，有很好的比例 养殖网箱与实验网箱的扩群比例为1：6，一般形成一个以上的网箱群 而不是50个笼子。正如表1清楚地显示的那样，不可能由每个个体建立如此多的殖民地 研究人员和列出的许多鼠标品系将在两个或更多不同的实验室使用。因此，斯蒂芬 将指导老鼠饲养的动物技术员齐富尼将能够协调 几个平行实验室的同一种基因的幼崽。团队合作也会将损失降至最低。一些人 实验设计需要特定性别的动物。然后，异性的幼崽就会 最有可能的是在不同实验室的另一项研究中找到用处，而不会被牺牲。 随着该计划的发展和项目在使用MICE方面的扩大，很明显，现在并不是所有的MICE 项目2(Hartwig)和项目4(Silberstein)所需的设施可安置在CBRI。因此只有他们的老鼠才会 在CBRI协作使用，用于成像、血栓形成研究、血小板清除、归巢 实验等将被安置在CBRI内，而其余的小鼠则需要进行非合作研究 因为这两个项目将根据分包合同安置在各自的内部设施中。核心将继续 以保持在所有4个项目中较少使用的菌株。在CBRI生产的小鼠很容易被转移到 其他机构使用CBRI面包车，为此我们一直为我们的动物保存有效的健康证书 立案了。 瓦格纳博士的实验室成员培训了一名技术专家斯蒂芬·齐富尼，他是波士顿学院生物学学士， 2004年。齐福尼先生已经在血液研究中心工作了几个月，已经 是这个项目的一部分。他现在将在核心B指导动物工作。他完全有能力管理 动物群体将继续是这个核心B的一部分。他的职责是并将继续是 1)早上第一件事就是检查所有笼子的水和食物供应，动物的清洁和健康。 立即纠正所有观察到的问题，并在必要时与护理人员交谈。 2)用红牌标记需要兽医注意的笼子，并联系兽医顾问Dr。 加里波迪，如果有必要的话。 3)对动物身体进行解剖，并采集患病动物的血液样本进行分析。准备好一套工具 进行培养，并在需要时成为兽医助手。 4)派出哨兵动物，做好记录。 5)根据要求安排饲养，并将动物运送到实验室。CBRI有一辆面包车，可以在以下情况下使用 实验室在另一栋楼里。 6)保存所有饲养和所有家养动物的每种基因的计算机记录。 7)指导所有新程序人员关于鼠标设施的程序和规则--包括 免疫缺陷室。 8)保持预算问题的记录，这样没有一个小组超支分配给他们的项目的金额。 9)积极参与CBRI的动物保护和使用委员会，起草和审查动物规程 特别是本计划所需的内容。 Cifuni先生还接受过特殊动物程序的培训，如眼眶后出血；尾随小鼠 基因分型；尾部DNA的PCR和Southern分析；组织学固定组织；尾静脉注射；等等。 能够向计划中的其他成员传授这些步骤

Animal models allowing investigation of the function of adhesion molecules in transfusion biology are one of the central themes of this program. Without any doubt the technology of genetic engineering of animals is one of the greatest advances in biology in recent years. Unfortunately, animal experimentation is not simple. In particular, there is variability: genetically engineered animals are not truly clones of each other and therefore their responses to treatment may differ. This is especially the case if the mice are of mixed background and not fully backcrossed to a particular genetic background. To minimize variability, wild-type animals are used from the same heterozygous matings to have litter mates comparisons or at least from the same animal colony. Therefore, often both mutant and wild-type animals have to be housed. So in addition to the mutant strains listed in Table 1, there are several wild-type strains that we need to keep. To obtain statistically meaningful results, each experiment has to involve a minimum of 6-10 animals of each genotype. The cost of animal housing is becoming prohibitive. This core unit is one of the reasons why this program was originally put together. To be able to maintain and breed a large number of mice of a certain genotype for experimental purposes, a mouse colony consisting of three breeding cages (to maintain the line) plus additional breeding and experimental cages (for expansion) is needed. In our experience a good ratio of breeding cages to experimental cages in the expansion colony is 1:6, commonly resulting in a colony of more than 50 cages. As Table 1 clearly shows this many colonies could not be established by each individual investigator and many mouse lines listed will be used in two or more different laboratories. Therefore, Stephen Cifuni, the animal technologist who will direct the mouse husbandry will be able to coordinate production of pups of one genotype for several laboratories in parallel. Working as a group will also minimize losses. Some experimental designs require a particular sex of the animals. The litter mates of the opposite sex will then most likely find use in another study at a different laboratory and will not have to be sacrificed. As the program developed and the projects expanded in their use of mice it became clear that not all mice now needed for Project 2 (Hartwig) and 4 (Silberstein) could be housed at CBRI. Therefore only their mice that will be used collaboratively at CBRI for imaging purposes, thrombus formation studies, platelet clearance, homing experiments etc will be housed within CBRI while the rest of the mice required for non -collaborative studies for these two projects will be housed in their own in-house facilities under subcontracts. The Core will continue to maintain strains that are used more rarely for all 4 projects. Mice produced at CBRI are easily transferred to the other institutions using the CBRI van and for this we keep a valid health certificate for our animals always on file. Dr. Wagner's laboratory members have trained a technologist, Stephen Cifuni, B.S. in Biology, Boston College, 2004. Mr. Cifuni has been employed at the Center for Blood Research for several months and has already been part of this program. He will now be directing the animal work in Core B. He is fully capable of managing the animal colonies that will continue to be part of this Core B. His duties are and continue to be the following 1) Check all cages first thing in the morning for water and food supplies, cleanliness and health of the animals. Correct all observed problems immediately and speak to care givers as necessary. 2) Mark cages that require veterinary attention with red cards and contact the veterinary consultant, Dr. Garibaldi, if necessary. 3) Make autopsies on dead animals and collect blood samples from sick animals for analysis. Have a kit ready to make cultures and be a veterinary helper as needed. 4) Send out sentinel animals and keep records. 5) Set up breedings on request and transport animals to laboratories. CBRI has a van that can be used if laboratory is located in another building. 6) Keep computerized records of all breedings and of all housed animals for every genotype. 7) Instruct all new program personnel on procedures and rules of the mouse facility - including the immuno-deficient room. 8) Keep records on budgetary issues, so that no group overspends the amount allocated to their project. 9) Actively participate in the Animal Care and Use Committee at CBRI, writing and reviewing animal protocols in particular those needed for this Program. Mr. Cifuni is also trained in special animal procedures such as retro-orbital bleeding; tailing mice for genotyping; PCR and Southern analysis of tail DNA; fixing tissues for histology; tail vein injections; etc. He is able to teach other members of the program these procedures