La Jolla Interdisciplinary Neuroscience Center Cores - Structural Bio Core Salk

结构生物岩心 1.主要目标和新方向 结构生物学在La Jolla Torrey Pines台地上的应用已经大大增加了 在过去的5年里。例如，BIMR增加了4名新的教职员工，成为其主要用户 结构生物学设施，由结晶学罗伯特·利丁顿指导。然而，目前 这个核心是伯纳姆国立卫生研究院资助的癌症中心的一部分，很少或根本无法接触到神经科学家 在La Jolla Torrey Pines台地。此外，索尔克大学的两名教员也投入了大量资金 但到目前为止，结晶学并没有以核心的形式组织起来，因此与 神经学家。此外，加州大学圣迭戈分校核磁共振负责人斯坦·奥佩拉在这一领域享有盛誉 并有一个核磁共振设备，将作为卫星提供给神经科学家。 核心。因此，结构核心将与设在BIMR的总部和设在 索尔克研究所和加州大学圣地亚哥分校。这种结构核心将通过允许平等的 接触神经学家。BIMR在结构生物学仪器方面进行了重大投资， 包括购置三台新的核磁共振谱仪和升级X射线衍射仪 向上。加州大学圣迭戈分校安装了一台最先进的900兆赫光谱仪，将用于 神经学家的合作。在最近的过去，科学进步是令人印象深刻的，因为这些 设施，包括在《细胞》、《科学》和《自然》杂志上发表的6篇论文，这些论文利用了来自 这些设施。然而，神经科学家几乎没有机会接触到教职员工或 仪器，因为它们位于BIMR、TSRI、UCSD和 索尔克。目前的拨款将通过提供设施和教师来纠正这一点 以神经科学家为核心单位。 总而言之，结构生物学核心包括两个设施：结晶学和核磁共振。 科学、后勤和预算问题为这种服务合并提供了理由。初级阶段 结晶学和核磁共振的应用是重叠的，大多数研究项目都需要两者 结晶学和核磁共振。合并后的设施满足了用户的需求，拥有广泛的 专业知识。有几位神经科学研究者不是主流的结构生物学家 而是那些渴望学习结构技术的人。该设施提供适当的培训和 为这些人指明方向。其他“核心”结构团体需要的支持要少得多，而且 设施将主要提供必要的硬件和技术支持，以实现科学 目标。一位经理(他可以免费获得这笔拨款)负责监督结构生物学中的两项技术 设施，并提供必要的培训(与教师主管一起)；技术人员 由当前赠款支付的资金将协调三个中心(BIMR、Salk、UCSD和 也促进了TSRI神经科学家的访问)。未来的计划包括在这里建立一个蛋白质表达设施 核心。

STRUCTURAL BIOLOGY CORE 1 . MAIN OBJECTIVES AND NEW DIRECTIONS The use of Structural Biology on the La Jolla Torrey Pines Mesa has increased tremendously over the past 5 years. For example, BIMR has added 4 new faculty members as major users of its Structural Biology Facility and is directed by crystallography Robert Liddington. However, presently this Core is part of the Burnham NIH-funded Cancer Center with little or no access to neuroscientists on the La Jolla Torrey Pines Mesa. Additionally, two faculty at The Salk are also heavily invested in crystallography but heretofore were not organized as a Core and thus have little interaction with neuroscientists. Moreover, the Head of NMR at UCSD, Stan Opella, is world-renowned in this area and has an NMR facility that would be made available to neuroscientists on this grant as a satellite core. Hence, the structural core will function with headquarters at BIMR and satellite facilities at The Salk Institute and UCSD. This Structural Core will foster collaborative interactions by allowing equal access to neuroscientists. BIMR has made major investments in instrumentation for structural biology, including the acquisition of three new NMR spectrometers and upgrades for the X-ray diffraction set up. UCSD has installed a state-or-the-art 900 MHz spectrometer, which will be available for collaborations of neuroscientists. Scientific progress has been impressive in the recent past by these facilities, including 6 papers in the journals Cell, Science and Nature that utilized structural data from the facilities. However, Neuroscientists have had minimal, if any, access to the faculty or instrumentation because they are located within the Cancer Centers at BIMR, TSRI, UCSD, and The Salk. The current grant will rectify this by making the facility and the faculty available to Neuroscientists as a Core Unit. In summary, the Structural Biology Core comprises two facilities: Crystallography and NMR. Scientific, logistical, and budgetary issues provide justification for this merger of services. The primary application of Crystallography and NMR overlap, and most research projects require both Crystallography and NMR. The merged facility serves the needs of users with a broad range of expertise. There are several neuroscience investigators who are not mainstream structural biologists but who have a desire to learn structural techniques. The facility provides appropriate training and direction for these people. Other "hardcore" structural groups require much less support, and the facility will primarily supply the necessary hardware and technical support to achieve the scientific goals. One manager (who is free to this grant) oversees both techniques within the Structural Biology Facility and provides the necessary training (in conjunction with the faculty supervisors); technicians paid for by the current grant will coordinate the efforts at the three centers (BIMR, Salk, UCSD, and facilitate TSRI neuroscientist access as well). Future plans include a protein expression facility in this Core.