In Vivo Core - Interdepartmental Two-photon Imaging Center

NU和FSM对共享2P成像设施的发展做出了坚定的承诺。密克罗尼西亚联邦有 投资约220万美元，用于购买设备、翻新场地和聘请技术人员 三个现有的2P核心设施。作为他们持续承诺的一部分，密克罗尼西亚联邦已授权分配 额外30万美元，用于购买仪器，以创建用于体内2P成像的核心(Core 4)。在最初的P30申请中，这是一项与之匹配的承诺，但这笔钱是出于善意 尽管未能获得NINDS支持，但仍做出手势。值得注意的是，250万美元的数字没有反映 学校对新教师的投资(工资、实验室翻新、启动成本)，以及在以下方面的专业知识 2P成像应用程序(Grutzendler博士、Waters博士、Shepherd博士和Osten博士)；这项投资超过400万美元。如果 其中一项增加了过去三年对从事树突-突触生理学和神经学研究的教师的投资 干细胞生物学(创建神经科学社区，在其中这些技术可以进行氟化)，总NU 对这家企业的承诺超过2500万美元。有了这项提案所要求的支持，我们可以在 这一承诺是通过改善现有核心的运作以及为非国家创新发展中心创建新的核心而得到资助的 需要这些技术来推进其研究计划但无法满足 现在时。如果NINDS采取这一步骤，提供更多空间(核心)，NU准备扩大他们的承诺 5和6)和核心工作人员(Wokosin博士、Hockberger博士和Core 6工作人员；见所附承诺 字母)。

NU and FSM have made a strong commitment to the development of shared 2P imaging facilities. FSM has invested approximately $2.2M to purchase equipment, renovate space and hire technical staff for the three existing 2P core facilities. As part of their continuing commitment, FSM has authorized the allocation of an additional $300K for the purchase of instrumentation to create a core for in vivo 2P imaging (Core 4). This was a 'matching' commitment in the original P30 application but the money was given as a good faith gesture in spite of the failure to secure NINDS support. It is worth noting that the $2.5M figure does not reflect the school's investment in new faculty (salaries, lab renovations, start-up costs) with demonstrated expertise in 2P imaging applications (Drs. Grutzendler, Waters, Shepherd, and Osten); this investment exceeds $4M. If one adds in the investment in the last three years in faculty working in dendritic-synaptic physiology and neural stem cell biology (creating the neuroscience community in which these techniques can fluorish), the total NU commitment to this enterprise exceeds $25M. With the support called for in this proposal, we can build on this commitment by improving the operation of the existing cores as well as creating new ones for NINDS funded investigators who need these technologies to advance their research programs but cannot be accommodated at present. NU is prepared to expand their commitment if NINDS takes this step, providing additional space (Cores 5 and 6) and salary support for core staff (Drs. Wokosin, Hockberger and Core 6 staff; see accompanying commitment letters).