SEPARATIONS/STRUCTURE/BIOAVAILABILITY/KRAUS, GEORGE

分离/结构/生物利用度核心将为提取、分馏和 植物化学物质的结构测定。核心将建立在克劳斯获得的经验基础上 在植物化学物质的标准和结构鉴定的制定方面的小组加上经验 由Murphy小组在紫锥菊和金丝桃样品的提取和分级中获得。 将密切进行提取、分离和结构测定/标准制备 与中心内的个别项目建立伙伴关系。将对生物活性进行生物利用度研究 以确定这些化学物质是否被吸收并达到相关的细胞靶点。 我们的目标是： 1.为项目研究人员提供金丝桃、紫锥菊、紫锥菊等药用植物提取物 夏枯草。超声波和索氏提取法将进行比较，以确定哪种方法 方法提供了最有用的提取。 2.分级生物活性提取物，以确定活性是分成特定的组分还是多个组分。 在高效液相色谱上收集感兴趣的跨梯度化合物将是主要的分馏策略。 3.确定药用植物各成分的结构。光谱学的组合 方法(红外光谱、核磁共振、质谱学) 4.提供化学指纹识别和检测的标准。中药复方标准品的研制 将基于合成紫锥菊烷胺的新方法- 克劳斯实验室开发的相关酮和原花青素合成专利方法。其他 将根据需要开发具有重要生物活性的化合物的合成方法。 5.测定提取物、组分和成分的生物利用度。Caco-2细胞将被用作模型 对人体肠道对感兴趣的草药化合物的摄取和代谢，以及 可能改变化合物摄取的部分。葡萄糖醛酸偶联物预计将是主要的和次要的 草药酚类的生物活性代谢物.草药成分对葡萄糖醛酸代谢的诱导性 也将进行研究。

The Separations/Structure/Bioavailability core will provide a resource for the extraction, fractionation and structural determination of phytochemicals. The core will build upon the experience gained by the Kraus group in the preparation of standards and structural identification of phytochemicals plus the experience gained by the Murphy group in the extraction and fractionation of Echinacea and Hypericum samples. Extraction, fractionation and structural determination/standard preparation will be conducted in close partnership with individual projects within the Center. Bioavailability studies will be conducted on bioactive compounds to determine if these chemicals are absorbed and reach relevant cellular targets. Our objectives are to: 1. Provide project researchers with extracts of medicinal plants including Hypericum, Echinacea and Prunella vulgaris. Ultrasonication and Soxhlet extraction methods will be compared to determine which method provides the most useful extracts. 2. Fractionate bioactive extracts to determine if activities separate into specific or multiple fractions. Collection of compounds of interest across gradients on HPLC will be a primary fractionation strategy. 3. Determine the structures of individual components of medicinal plants. A combination of spectroscopic methods (IR, NMR, mass spectroscopy) 4. Provide standards for chemical fingerprinting and testing. The preparation of herbal compound standards that are not commercially available will be based upon novel methods for synthesizing Echinacea alkamide- related ketones and a patented method for procyanidin synthesis developed by the Kraus laboratory. Other synthetic approaches will be developed as needed for compounds of important bioactivity. 5. Determine the bioavailability of extracts, fractions and components. CaCo-2 cells will be used as a model for human intestinal uptake and metabolism of herbal compounds of interest, as well as interactions between fractions that may alter compound uptake. Glucuronide conjugates are expected to be major and less bioactive metabolites from herbal phenolics; the inducibility of glucuronide metabolism by herbal components will also be studied.