SGER: Development of a Reporter System Regulated by the Biological Availability of Fe in Saltwater
SGER: Development of a Reporter System Regulated by the Biological Availability of Fe in Saltwater
批准号:
0002968
负责人:
Steven Wilhelm
金额:
$4.82万
依托单位国家:
美国
项目类别:
Standard Grant
财政年份:
2000
资助国家:
美国
项目状态:
已结题
起止时间:
2000-06-01 至 2002-05-31
中文摘要
铁(Fe)作为海洋系统生物生产力的限制因子的作用已被广泛研究。已知海洋原核生物在限制铁的生长条件下保持产生铁载体(或至少类似铁载体的化合物)的能力。虽然研究分析技术可以在非常小的浓度下量化总铁,并提供有关铁形态的信息,但我们仍然没有这种铁对自然群落的生物有效性的指标。为了了解铁在全球海洋生产力中的作用,需要量化真正生物可利用铁的工具/技术。由于水生生物可以确定自身的铁状态,因此基于海洋生物铁感知系统的工具将是最好的。研究人员已经开发出一种具有发光报告的基因工程微生物(GEM),它使用海洋细菌的天然铁摄取调节蛋白(Fur)直接响应铁的生物利用度。研究人员现在将尝试将报告菌整合到其他10种海洋细菌中。实验室实验将建立铁生物利用度和光产量之间的关系。GEMs作为铁生物利用度报告器的现场测试将在马尾藻海、伊利湖和秘鲁上升流地区进行。
英文摘要
The role of iron (Fe) as a limiting agent of biological productivity in marine systems has been studied extensively. Marine prokaryotic organisms are known to maintain the ability to produce siderophore (or at least siderophore-like compounds) in response to Fe-limiting growth conditions. While research analytical techniques can quantify total Fe at incredibly small concentrations and provide information on Fe speciation, we remain without indicators of the biological availability of this Fe to natural communities. To understand the role that Fe plays in global marine productivity, tools/techniques for quantifying truly bioavailable iron are needed. Since organisms in aquatic systems can determine their own Fe status, a tool based on the Fe sensing system of a marine organism would be the best. The investigators have developed a genetically engineered microorganism (GEM) with a luminescent reporter that directly responds to Fe bioavailability using the native ferric-uptake-regulator (Fur) protein of a marine bacterium. The researchers will now attempt integration of the reporter into ten other strains of marine bacteria. Laboratory experiments will establish the relationship between Fe bioavailability and light production. Field testing of the GEMs as Fe bioavailability reporters will be conducted on cruises of opportunity in the Sargasso Sea, Lake Erie, and the Peru upwelling region.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
REU Site: Microbial community interactions and functions
-
批准号:2050743
-
项目类别:Continuing Grant
-
资助金额:$40.23万
-
财政年份:2021
-
负责人:Steven Wilhelm
-
依托单位:
EDGE CT: Genetic tools to study giant viruses
-
批准号:1922958
-
项目类别:Standard Grant
-
资助金额:$100.93万
-
财政年份:2019
-
负责人:Steven Wilhelm
-
依托单位:
Collaborative Research: Inferring Cellular Lysis and Regeneration of Organic Matter by Marine Viruses
-
批准号:1829641
-
项目类别:Continuing Grant
-
资助金额:$59.84万
-
财政年份:2018
-
负责人:Steven Wilhelm
-
依托单位:
REU Site: Microbial community interactions and functions
-
批准号:1659599
-
项目类别:Standard Grant
-
资助金额:$34.04万
-
财政年份:2017
-
负责人:Steven Wilhelm
-
依托单位:
Collaborative Research: An Integrated Approach to Understanding the Function of the Potent Hepatotoxin Microcystin in the Growth & Ecology of Microcystis
-
批准号:1451528
-
项目类别:Continuing Grant
-
资助金额:$71.94万
-
财政年份:2015
-
负责人:Steven Wilhelm
-
依托单位:
Dimensions: Collaborative Research: Anthropogenic nutrient input drives genetic, functional and taxonomic biodiversity in hypereutrophic Lake Taihu, China
-
批准号:1240870
-
项目类别:Standard Grant
-
资助金额:$56.3万
-
财政年份:2013
-
负责人:Steven Wilhelm
-
依托单位:
REU Site: Microbial community interactions and functions
-
批准号:1156644
-
项目类别:Standard Grant
-
资助金额:$31.49万
-
财政年份:2012
-
负责人:Steven Wilhelm
-
依托单位:
Collaborative Research: Characterizing the Constraints on Virus Infection of Cyanobacteria
-
批准号:0851113
-
项目类别:Standard Grant
-
资助金额:$29.98万
-
财政年份:2009
-
负责人:Steven Wilhelm
-
依托单位:
What Makes Microcystis Bloom? Dissecting the Physiological Ecology of a Toxic Cyanobacterium with Community Level Proteomics
-
批准号:0841918
-
项目类别:Standard Grant
-
资助金额:$45.87万
-
财政年份:2009
-
负责人:Steven Wilhelm
-
依托单位:
Collaborative Proposal: Decoding Virus Leviathans
-
批准号:0949120
-
项目类别:Standard Grant
-
资助金额:$27.5万
-
财政年份:2009
-
负责人:Steven Wilhelm
-
依托单位:
Collaborative Research: Evaluating Nutrient Reductions to Control Cyanobacteria and Ensure Large Lake Sustainability: Lake Taihu (China) as a Model for North American Systems
-
批准号:0826838
-
项目类别:Continuing Grant
-
资助金额:$10.16万
-
财政年份:2008
-
负责人:Steven Wilhelm
-
依托单位:
Collaborative Research: FeCycle I I- Natural variability in plankton iron quotas during an unamended Lagrangian experiment
-
批准号:0825405
-
项目类别:Standard Grant
-
资助金额:$17.43万
-
财政年份:2008
-
负责人:Steven Wilhelm
-
依托单位:
Viral Abundance, Production and Diversity During the North Atlantic Spring Bloom (NASB 2005)
-
批准号:0452409
-
项目类别:Standard Grant
-
资助金额:$35.73万
-
财政年份:2005
-
负责人:Steven Wilhelm
-
依托单位:
Development of Bioluminescent Bacterial Bioreporters to Quantify the Bioavailability of Fe in Seawater
-
批准号:0526159
-
项目类别:Standard Grant
-
资助金额:$46.61万
-
财政年份:2005
-
负责人:Steven Wilhelm
-
依托单位:
U.S.-New Zealand Collaborative Research: The Biogeochemical Cycling of Iron in Waters of the Subantarctic Southern Ocean (FeCycle)
-
批准号:0240092
-
项目类别:Standard Grant
-
资助金额:$0.97万
-
财政年份:2003
-
负责人:Steven Wilhelm
-
依托单位:
SGER: Viral Dynamics and the Southern Ocean Fe-cycle
-
批准号:0228895
-
项目类别:Standard Grant
-
资助金额:$2.27万
-
财政年份:2002
-
负责人:Steven Wilhelm
-
依托单位:
Viral Influence on Freshwater Cyanobacteria and Toxin Dynamics
-
批准号:0129118
-
项目类别:Standard Grant
-
资助金额:$30.01万
-
财政年份:2002
-
负责人:Steven Wilhelm
-
依托单位:
SGER: Viral Impacts on Fluvial and Limnetic Biogeochemistry
-
批准号:0003069
-
项目类别:Standard Grant
-
资助金额:$3.49万
-
财政年份:2000
-
负责人:Steven Wilhelm
-
依托单位:
Bioavailability of Carbon and Iron from Viral Lysis Products
-
批准号:9977040
-
项目类别:Standard Grant
-
资助金额:$24.31万
-
财政年份:1999
-
负责人:Steven Wilhelm
-
依托单位:
国内基金
海外基金
水稻边界发育缺陷突变体abnormal boundary development(abd)的基因克隆与功能分析
-
批准号:32070202
-
项目类别:面上项目
-
资助金额:58.0万元
-
批准年份:2020
-
负责人:汪泉
-
依托单位:
Development of a Linear Stochastic Model for Wind Field Reconstruction from Limited Measurement Data
-
批准号:--
-
项目类别:--
-
资助金额:40万元
-
批准年份:2020
-
负责人:Vikrant Gupta
-
依托单位: