Animal Core

核心C（动物核心）的主要作用是提供个体所需的适当动物 项目中的项目。动物核心将提供三个基本支持功能。一是 动物中心将负责维持项目1所需的ETe受体缺陷大鼠群 和2，项目3和4所需的细胞特异性敲除小鼠，以及适当的对照集落。 第二，Animal Core将负责产生收集管特异性双敲除小鼠 在项目3中使用。第三，动物中心将提供平均动脉的连续测量 使用Data Sciences遥测系统，在所有要求的大鼠和 项目1、2和3中的小鼠研究。目的1.建立ETe受体缺陷大鼠模型， 胆管NOSI敲除小鼠。这将包括建立适当的繁殖对，收集组织， 与核心B协调进行基因分型，并确保有足够数量的动物可用于 个别项目。这些动物模型将由位于 格鲁吉亚医学院。目的二是提供集合管ET-1、ETA和ETB受体的基因敲除方法 小鼠这将包括建立适当的育种对，基因分型，并与 格鲁吉亚医学院和德克萨斯大学健康科学中心提供足够的数字 动物为个别项目。这些小鼠将由犹他州大学提供。目标3是 提供集合管NOS 1/ET-1双基因敲除小鼠。这将包括育种floxed NOSI，floxed ET-1和水通道蛋白-2（AQP 2）-Cre小鼠。目前正在为项目3制定这些标准。目标4：提供 连续测量小鼠和大鼠的平均动脉压、心率和体力活动， 格鲁吉亚医学院。这将包括调度，并提供材料，手术，和日常 在整个研究过程中对各个项目进行监测。动物核心的结构是为了提供 最高程度的质量控制，并允许所有个体动物模型的一致性 计划项目的项目。这将涉及非常密切的协调努力和分享专门知识 在犹他州和格鲁吉亚医学院的研究小组之间。

The primary role of Core C (the Animal Core) is to provide the appropriate animals required by the individual projects within the Program Project. The Animal Core will serve three basic support functions. First, the Animal Core will be responsible for maintaining the ETe-receptor deficient rat colony required by Projects 1 and 2, the cell-specific knockout mice required by Projects 3 and 4, and the appropriate control colonies. Second, the Animal Core will be responsible for generating the collecting duct-specific double knockout mice used in Project 3. Third, the Animal Core will provide for the continuous measurement of mean arterial pressure, heart rate and physical activity using the Data Sciences telemetry systems in all required rat and mouse studies in Projects 1, 2, and 3. Aim 1 is to provide the ETe-receptor deficient rats and the collecting duct NOSI knockout mice. This will include setting up the appropriate breeding pairs, collecting tissue and coordinating with Core B for genotyping, and ensuring that a sufficient number of animals are available for the individual projects. These animal models will be provided by the section of the Core located at the Medical College of Georgia. Aim 2 is to provide the collecting duct ET-1, ETA and ETB receptor knockout mice. This will include setting up the appropriate breeding pairs, genotyping, and coordinating with the Medical College of Georgia and the University of Texas Health Science Center to provide sufficient numbers of animals for the individual projects. These mice will be provided by the University of Utah. Aim 3 is to provide the collecting duct N0S1/ET-1 double knockout mice. This will include breeding floxed NOSI, floxed ET-1 and aquaporin-2 (AQP2)-Cre mice. These are being developed for Project 3. Aim 4 is to provide continuous measurement of mean arterial pressure, heart rate and physical activity in mice and rats at the Medical College of Georgia. This will include scheduling, and providing materials, surgery, and daily monitoring throughout the study for the individual projects. The Animal Core has been structured to provide the highest degree of quality control and allow for consistency in animal models across all of the individual projects of the Program Project. This will involve very closely coordinated efforts and sharing of expertise between the groups at the University of Utah and the Medical College of Georgia.