Molecular and Cellular Biology Core

核心C包括主任以及一名技术人员，他将提供分子和细胞生物学 支持本计划项目建议书中的三个项目。该核心将提供三大 这些功能将协助每个实验室进行实验，目的是：1）操纵 体外和体内血管平滑肌细胞内的基因表达; 2）产生和操纵 将重组蛋白应用于容器或细胞;和3）从野生型或重组蛋白中分离细胞。 敲除突变动物，并将它们在二维或三维细胞外培养， 环境.核心总监在重组病毒载体的创建方面有相当丰富的经验 用于将基因递送到血管细胞中以及siRNA技术来抑制原发性 血管细胞包括血管平滑肌细胞、周细胞和内皮细胞。另外核心 主任在培养和操作原代血管细胞方面具有丰富的经验， 或三维文化。这一核心还将提供一个重要的功能， 研究人员将推进微血管内基因表达技术， 微循环功能主任将参加所有与提案有关的科学活动， 能够适当地满足每个项目的需要，并促进每个研究的分子方面 方向 这一核心将提供服务，这些服务对以下方面的整体功能和科学整合至关重要： 计划项目补助金。所有项目都将依赖于该核心在每个国家提供的服务， 上述三个重点领域。

Core C consists of the director as well as one technician who will provide molecular and cellular biology support for each of the three projects in this program project proposal. This core will provide three major functions which will be to assist each ofthe laboratories in experiments designed to; 1) manipulate gene expression within vascular smooth muscle cells in vitro and in vivo; 2) produce and manipulate recombinant proteins to be applied to vessels or cells; and 3) to isolate ceils from wild-type or knockout mutant animals and to culture them in two-dimensional or three-dimensional extracellular environments. The core director has considerable experience in the creation of recombinant viral vectors for gene delivery into vascular cells as well as siRNA technology to suppress gene expression in primary vascular cells including vascular smooth muscle cells, pericytes and endothelial cells. In addition, the core director has extensive experience in the culture and manipulation of primary vascular cells in either twodimensional or three-dimensional culture. This core will also provide an important function to facilitate each investigator to advance gene expression technologies within microvessels to be able to manipulate microcirculatory function. The director will participate in all scientific functions related to the proposal to be able to properly serve the needs of each project and to facilitate the molecular aspects of each research direction. This core will provide services that are essential for the overall function and scientific integration of the Program Project Grant. All projects will rely on the services provided by this core in each of the three main areas of focus described above.