P3-ADULT BONE MARROW STEM CELLS FOR CNS REPAIR

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 来自骨髓的成体干细胞有可能为神经系统疾病提供引人注目的新疗法。 除了干细胞直接替代细胞外，目前的数据提供了证据表明，成体干细胞分泌到受损CNS环境中的神经营养因子和生长因子可能为神经系统修复提供强大的刺激。 我们的工作表明，骨髓干细胞移植的主要作用是刺激脑内内源性CNS祖细胞的增殖。 我们已经提出了一系列的实验来测试中心的假设，即来自人骨髓的成体干细胞的亚群可以有效地植入并通过影响内源性CNS祖细胞的增殖和分化来修复受损的脑。 具体目标是： 1.建立人骨髓成体干细胞亚群的分子特征，并与中枢神经系统祖细胞进行比较。 2.确定不同人干细胞亚群移植后内源性CNS祖细胞的增殖和分化模式。 3.目的：评价干细胞移植治疗脑卒中动物后对中枢神经系统祖细胞的反应。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Adult stem cells from bone marrow have the potential to provide dramatic new therapies for neurological disorders. In addition to direct cell replacement by stem cells, current data provide evidence that neurotrophins and growth factors secreted by adult stem cells into the damaged CNS environment may provide a powerful stimulus for nervous system repair. Our work demonstrates that the primary effect of transplanted bone marrow stem cells is to stimulate the proliferation of endogenous CNS progenitors in the brain. We have proposed a series of experiments to test the central hypothesis that sub-populations of adult stem cells from human bone marrow can effectively engraft and repair the injured brain by influencing the proliferation and differentiation of endogenous CNS progenitors. The Specific Aims are: 1. To establish the molecular profiles of purified adult stem cell sub-populations from human bone marrow and compare them to CNS progenitor cells. 2. To determine the proliferation and differentiation patterns of endogenous CNS progenitors following the transplantation of different human stem cell sub-populations. 3. To evaluate the response of CNS progenitor cells following stem cell transplantation in an animal injured by stroke.