P3-ADULT BONE MARROW STEM CELLS FOR CNS REPAIR

用于中枢神经系统修复的 P3-成人骨髓干细胞

• 批准号: 7725302
• 负责人: JEFFREY L SPEES
• 金额: $ 24.8万
• 依托单位: UNIVERSITY OF VERMONT & ST AGRIC COLLEGE
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-07-01 至 2009-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7725302
• 关键词: Adult; Affect; Bone Marrow; Bone Marrow Stem Cell; Brain; Cells; Central Nervous System Diseases; Clinical; Computer Retrieval of Information on Scientific Projects Database; Data; Disease; Engraftment; Funding; Grant; Growth Factor; Institution; Ischemic Stroke; Literature; Marrow; Mediation; Mus; Neuraxis; Play; Population; Reporting; Research; Research Personnel; Resources; Role; Series; Source; Standards of Weights and Measures; Stem cells; Testing; Therapeutic; Transplantation; United States National Institutes of Health; adult stem cell; cell injury; neuronal survival; neuroprotection; neurotrophic factor; paracrine; repaired; research study; stem

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Adult stem cells from bone marrow have the potential to provide powerful new treatments for central nervous system (CNS) disorders. Currently it is unclear which cells or sub-populations will be best suited to effectively treat a variety of diseases that may be amenable to such a therapeutic approach. Furthermore, in addition to the standard paradigm of stem cell engraftment, differentiation, and replacement of damaged cells, several reports in the literature, as well as our preliminary data, suggest that the secretion of neurotrophic factors by transplanted adult stem cells may play the principal role in their mediation of CNS repair. The identification of a bone marrow-derived stem cell sub-population that is best suited for neuroprotection or repair would be of great potential clinical importance. We have proposed a series of experiments to test the central hypothesis that an optimized population of paracrine-acting adult stem cells from bone marrow can be used for CNS repair. The specific aims are: 1. To determine whether specific populations of adult bone marrow stem cells secrete growth factors with known neurotrophic activities. 2. To determine whether transplantation of the "high neurotrophin population" of marrow stem cells activates endogenous mouse CNS cells, including endogenous stem cells. 3. To determine whether the "high neurotrophin population" of marrow stem cells affects neuronal survival and activates endogenous stem cells in brain after ischemic stroke.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 来自骨髓的成体干细胞有可能为中枢神经系统（CNS）疾病提供强大的新治疗方法。 目前尚不清楚哪种细胞或亚群最适合有效治疗可能适用于这种治疗方法的各种疾病。此外，除了干细胞移植、分化和替换受损细胞的标准范例之外，文献中的一些报告以及我们的初步数据表明，移植的成体干细胞分泌神经营养因子可能在其介导CNS修复中发挥主要作用。 鉴定最适合神经保护或修复的骨髓源性干细胞亚群将具有巨大的潜在临床意义。 我们已经提出了一系列的实验来测试中心的假设，一个优化的人口旁分泌作用的成体干细胞从骨髓中可以用于中枢神经系统的修复。 具体目标是： 1. 确定特定群体的成人骨髓干细胞是否分泌具有已知神经营养活性的生长因子。 2. 确定骨髓干细胞的“高神经营养因子群体”移植是否激活内源性小鼠CNS细胞，包括内源性干细胞。 3.目的：探讨缺血性脑卒中后骨髓干细胞的高神经营养因子群是否影响神经元存活和激活内源性干细胞。