VECTOR-BORNE DISEASES CORE

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 VBD核心继续保持着一个斯氏按蚊群体，用于将食蟹猴疟原虫(一种人类间日疟的模型)传播给恒河猴。在使我们的部门能够利用自然感染模式对莱姆型疏螺旋体病进行研究方面，壁虱群体继续发挥着重要作用。我们现有的若虫毛细血管喂养技术，使我们能够用螺旋体克隆分离株感染壁虱。这通常是确保明确的宿主对感染的反应所必需的。我们也能够通过浸泡在含有螺旋体的组织培养液中感染幼虫。核心的扁虱部分目前有大量肩部硬蜱发育阶段的标本。幼虫、若虫和成虫以交错方式储存在4℃。因此，我们通常在一年中的大部分时间都有所有的舞台。这包括5-10瓶幼虫(每个约有1000只幼虫)和数百只未感染的若虫，以供实验需要。今年，我们继续收集扁虱唾液，研究其对伯氏杆菌入侵宿主皮肤时引起的炎症反应的影响。我们还继续与德克萨斯州休斯敦的德克萨斯大学健康科学中心合作，确定伯氏杆菌的毒力决定因素，以影响螺旋体对扁虱和通过扁虱对小鼠的传染性。今年收集了46只成年雌性，并进行了血液喂养。其中27只喂食成功，大多数产卵。取食和采集的幼虫数量为866头，其中660头成功蜕皮成若虫，供未来实验使用。共有194只若虫成功地被毛细管喂养。这些扁虱被用在三个大型的独立实验中。其余的仙女将在本年度使用。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The VBD core has continued to maintain an Anopheles stephensi colony, which is used to transmit Plasmodium cynomolgi, a model of human vivax malaria, to rhesus macaques. The tick colony has continued to be instrumental in enabling our division to do research in Lyme borreliosis using the natural mode of infection. The technique of capillary feeding of nymphal ticks, which we have available, allows us to infect ticks with spirochetal clonal isolates. This is often essential to insure defined host-responses to infection. We also are able to infect larval ticks by immersion in tissue-culture fluid that contains spirochetes. The tick section of the core has currently available numerous specimens of all of the developmental stages of Ixodes scapularis. Larvae, nymphs, and adults are stored at 4¿C in a staggered fashion. Therefore, we usually have all of the stages available at most times throughout the year. This includes 5-10 jars of larvae (with about 1000 larvae each) and several hundred uninfected nymphs for experimental needs as they arise. This year we have continued with the collection of tick saliva, to study its effects on the inflammatory response induced by B. burgdorferi spirochetes as they invade the host skin. We also have continued our collaboration with the University of Texas Health Sciences Center, Houston, TX, on the identification of virulence determinants of B. burgdorferi the affect infectivity of spirochetes to ticks, and to mice via ticks. This year 46 adult females were collected and were blood fed. A total of 27 of those fed successfully, and most laid eggs. The number of larvae that fed and were collected were 866, and 660 of those successfully molted into nymphs to be used in future experiments. A total of 194 nymphs were capillary-fed successfully. Those ticks were used in 3 large, separate experiments. The remainder of the nymphs will be used in the current year.