PATHOGEN DETECTION AND QUANTIFICATION CORE

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 病原体检测和定量核心（PDQC）是在基本赠款审查过程中批准该方案后于2003年底建立的。该核心旨在提供特定无病原体菌落的检测。核心目前正在运作。目前提供了SIV、STLV和B型疱疹感染的诊断测试。对于SIV和STLV，我们也提供确认检测。疱疹B的算法包括由PDQC进行筛查，然后在国家参考实验室进行确认。正在开发新的诊断测试并将其标准化。由于测试不同的肽形式没有提供良好的灵敏度，我们还优化了SRV的PCR筛选。 病毒诊断核心在2005年处理和检测了5 000多个样本，在2006年和2007年处理和检测了6 000多个样本。正在制定一项经济的样品处理新战略。2008年，为TNPRC殖民地的动物提供了10，000多项临床试验 我们目前正在探索B病毒和SRV诊断的新策略。预计到2009年底，将通过Luminex多重微珠技术进行常规检测。 实时PCR作为PDQC功能的一部分提供。 主要目标是：1.协助私家侦探使用该设施在这方面，所有以前使用rt-PCR核心的研究人员都得到了所需的支持。新的调查人员得到帮助，以执行他们的程序。2.提供病毒定量服务。我们优化了用于SIVmac/SHIV定量的rt-PCR测定。这种新的检测方法目前正在优化中。我们提供SIVagm和SIVrcm的定量测定。我们还提供基因定量（CCR 5，TGF-β，加塔-3，IL 6，IFN-γ）。2008年购买了一台新的7900序列放大器。2008年为8个项目提供了实时PCR定量。2008年，对血浆和组织进行了1000多次测定。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The pathogen detection and quantification core (PDQC) was established in late 2003 following the approval of the program during the base grant review process. This core is designed to supply testing for the specific pathogen free colonies. The core is currently functional. Diagnostic tests for SIV, STLV and Herpes B infections are currently provided. For SIV and STLV we are also providing confirmation assays. The algorithm for herpes B involves screening by the PDQC followed by confirmation in the National Reference laboratory. New diagnostic tests are being developed and standardized for SRV. Since testing different peptide formats did not offer a good sensitivity, we are also optimizing PCR screening for SRV. The viral diagnostic core processed and tested more than 5,000 samples in 2005 and over 6,000 in 2006 and 2007. A new strategy for economical sample processing is in progress. In 2008 over 10,000 clinical tests were provided for the animals in the TNPRC colony We are currently exploring new strategies for B virus and SRV diagnostic. It is expected that by the end of 2009 routine testing will be performed through the Luminex multiplex bead technology. Real-time PCR is offered as a part of capabilities of the PDQC. The major objectives are: 1. to assist PIs using the facility. In this respect, all of the investigators previously using the rt-PCR core received the needed support. New investigators were helped to implement their procedures. 2. To provide service for viral quantification. We optimized a rt-PCR assay for the SIVmac/SHIV quantification. This new assay is currently under optimization. We provide quantification assays for SIVagm and SIVrcm. We also provide genequantification (CCR5, TGF-beta, GATA-3, IL6, IFN-gamma). A new 7900 Squence amplifier was purchased in 2008. Real-time PCR quantification was provided in 2008 for 8 projects. Over 1000 assays have been carried out on both plasma as well as tissues in 2008.