OPTICAL IMAGING FACILITY

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 光学成像设施(OIF)于1999年秋季在UCC建立。我们的第一台主要设备使研究人员能够以快速的时间和空间分辨率评估细胞反应，如钙信号。根据本提案请拨的资金已用于为这一延时细胞成像系统提供技术支助，并维护其尖端设备。还要求提供资金，以支持将一个免疫细胞化学实验室并入法语国家组织。免疫细胞化学实验室提供了支持，并继续是产生高质量数据的重要基础设施，这些数据已在同行评议的期刊上发表。2007年，由于RCMI补充基金，OIF通过购买共焦显微镜扩大了服务范围。OIF目前的目标是： 1.为进行免疫细胞化学、定量荧光成像捕获和分析提供技术支持。 2.就如何有效地利用显微镜设备从活标本和固定标本中进行图像采集和处理提供仪器接触和培训。 3.通过为年轻教员提供获取初步数据的途径和支持，改善UCC的科学环境，帮助知名研究人员创新他们的研究，增加他们的出版记录，并通过组织设施用户之间的互动来加强合作。 光学成像设施的持续支持一直是细胞和分子生物学中心的一笔财富，因为它有助于提高现有参与者的生产力，并对潜在的新调查人员构成一个主要和有吸引力的研究资源。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Optical Imaging Facility (OIF) was established at UCC during the fall of 1999. Our first major equipment has enabled researchers to assess cellular responses such as calcium signaling with fast temporal and spatial resolutions. Funds requested under this proposal have been utilized to provide technical support for this time-lapsed cell imaging system and the maintenance of its sophisticated equipment. Funds were also requested to support the integration of an immunocytochemistry laboratory into the OIF. The immunocytochemistry laboratory has provided support and continues to be an important infrastructure to produce high quality data that has been published in peer-reviewed journals. In 2007, thanks to RCMI supplemental funds, the OIF expanded its offering through the acquisition of a Confocal Microscope. The current aims of the OIF are: 1. To provide technical support to perform immunocytochemistry, quantitative fluorescence imaging capture and analysis. 2. To provide instrument access and training on the effective utilization of microscope-based equipment to carry on image capture and processing from live and fixed specimens. 3. To enhance UCC's scientific environment by providing access and support to young faculty to obtain preliminary data, helping established investigators to innovate their research and increase their publication record and enhance collaboration by organizing interactions among the facility users. The continued support of the Optical Imaging Facility has been an asset to the Cell and Molecular Biology Center since it has contributed to enhance the productivity of its current participants and constitutes a primary and attractive research resource for potential new investigators.