OPTICAL IMAGING FACILITY

光学成像设备

• 批准号: 7715320
• 负责人: PRISCILA SANABRIA
• 金额: $ 13.26万
• 依托单位: UNIVERSIDAD CENTRAL DEL CARIBE
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-08-01 至 2009-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7715320
• 关键词: Calcium Signaling; Cells; Collaborations; Computer Retrieval of Information on Scientific Projects Database; Data; Data Quality; Environment; Equipment; Faculty; Funding; Grant; Image; Institution; Journals; Laboratories; Life; Maintenance; Microscope; Molecular Biology; Participant; Peer Review; Process; Productivity; Publications; Publishing; Research; Research Infrastructure; Research Personnel; Resolution; Resources; Source; Specimen; System; Time; Training; United States National Institutes of Health; base; cellular imaging; falls; fluorescence imaging; immunocytochemistry; innovation; instrument; optical imaging; response

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Optical Imaging Facility (OIF) was established at UCC during the fall of 1999. Our first major equipment has enabled researchers to assess cellular responses such as calcium signaling with fast temporal and spatial resolutions. Funds requested under this proposal have been utilized to provide technical support for this time-lapsed cell imaging system and the maintenance of its sophisticated equipment. Funds were also requested to support the integration of an immunocytochemistry laboratory into the OIF. The immunocytochemistry laboratory has provided support and continues to be an important infrastructure to produce high quality data that has been published in peer-reviewed journals. In 2007, thanks to RCMI supplemental funds, the OIF expanded its offering through the acquisition of a Confocal Microscope. The current aims of the OIF are: 1. To provide technical support to perform immunocytochemistry, quantitative fluorescence imaging capture and analysis. 2. To provide instrument access and training on the effective utilization of microscope-based equipment to carry on image capture and processing from live and fixed specimens. 3. To enhance UCC's scientific environment by providing access and support to young faculty to obtain preliminary data, helping established investigators to innovate their research and increase their publication record and enhance collaboration by organizing interactions among the facility users. The continued support of the Optical Imaging Facility has been an asset to the Cell and Molecular Biology Center since it has contributed to enhance the productivity of its current participants and constitutes a primary and attractive research resource for potential new investigators.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 光学成像设施 (OIF) 于 1999 年秋季在 UCC 建立。我们的第一台主要设备使研究人员能够以快速的时间和空间分辨率评估细胞反应，例如钙信号传导。 该提案要求的资金已用于为该延时细胞成像系统及其复杂设备的维护提供技术支持。还要求提供资金支持将免疫细胞化学实验室纳入 OIF。 免疫细胞化学实验室提供了支持，并将继续成为生成高质量数据的重要基础设施，这些数据已在同行评审的期刊上发表。 2007 年，得益于 RCMI 补充资金，OIF 通过收购共焦显微镜扩大了其产品范围。 OIF 目前的目标是： 1. 为免疫细胞化学、定量荧光成像捕获和分析提供技术支持。 2. 提供仪器使用和培训，以有效利用基于显微镜的设备对活体和固定标本进行图像捕获和处理。 3. 通过为年轻教师提供获得初步数据的机会和支持来改善 UCC 的科学环境，帮助成熟的研究人员创新他们的研究并增加他们的发表记录，并通过组织设施用户之间的互动来加强合作。 光学成像设施的持续支持一直是细胞和分子生物学中心的一项资产，因为它有助于提高现有参与者的生产力，并为潜在的新研究人员构成主要且有吸引力的研究资源。