OPTICAL IMAGING FACILITY

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。作为前一个由RCMI资助的周期的一部分，1999年秋季在UCC建立了一个光学成像设施。为该设施购买的第一台主要设备是基于显微镜的灵敏系统，该系统能够以快速的时间分辨率评估细胞反应，如钙信号。该系统还允许对这一功能变量进行空间评估，这意味着研究人员能够识别相邻细胞组中钙信号反应的异质性。该提案要求资金继续为这一延时细胞成像系统及其尖端设备的维护提供技术支持。由于实验的性质，这种特殊的显微镜装置是一个高度专用的系统。因此，为了为其他基于显微镜的应用提供技术支持，本提案还要求提供资金，以支持将一个免疫细胞化学实验室整合到光学成像设施中。免疫细胞化学实验室通过使用针对这些分子的特定抗体，专门对带有选择性标记的细胞进行定性鉴定和定位。参考实验室是在Serguei Skatchkov博士和Misty Eaton博士的倡议下于1999年组织的，他们在其项目的实验设计中成功地使用了这项技术。1997年，由于早期的RCMI支持的方法学转移计划，斯卡奇科夫博士在柏林洪堡大学的一个领先的免疫细胞化学实验室接受了培训。免疫细胞化学实验室设备包括立式荧光显微镜和高分辨率数码相机。该实验室获得的免疫细胞化学数据已发表在同行评议的期刊上，并在地方和国家研讨会上发表。目前，有几个项目将直接受益于这种基于显微镜的技术提供的服务。因此，光学成像设备的目标是：1.为时间和空间分辨率成像系统提供技术支持。将免疫细胞化学实验室整合到光学成像设备中。在我们的学术界传播我们设施的能力。继续支持光学成像设施将成为细胞和分子生物学中心的一项资产，因为它将有助于提高现有参与者的生产力，并将成为对潜在新的研究人员/合作者具有吸引力的研究资源。光学成像设备的免疫细胞化学能力将补充我们现有的成像平台，该平台配置用于快速获取和分析细胞内钙离子的荧光标记记者。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. As part of a previous funded RCMI cycle, an Optical Imaging Facility was established at UCC during the fall of 1999. The first major equipment acquired for this facility was a sensitive microscope-based system that allows the assessment of cellular responses such as calcium signaling with fast temporal resolution. The system also allows the spatial assessment of this functional variable, which means that an investigator is able to identify the heterogeneity of calcium signal responses in neighboring groups of cells. This proposal requests funds to continue providing technical support for this time-lapsed cell imaging system and the maintenance of its sophisticated equipment. Due to the nature of experiments, this particular microscope setup is a highly dedicated system. Therefore, in order to provide technical support for other microscope-based applications, this proposal is also requesting funds to support the integration of an immunocytochemistry laboratory into the Optical Imaging Facility. The immunocytochemistry laboratory specializes in the qualitative identification and localization of cells bearing selective markers by employing specific antibodies to these molecules. The referenced laboratory was organized in 1999 through the initiatives of Drs. Serguei Skatchkov and Misty Eaton, who have been successfully using this technology in the experimental design of their projects. In 1997, Dr. Skatchkov, thanks to an earlier RCMI supported Methodology Transfer Program, received training in a leading immunocytochemistry laboratory at Humboldt University, Berlin. The immunocytochemistry laboratory equipment includes an upright fluorescence microscope and a high-resolution digital camera. Immunocytochemistry data obtained in this laboratory has been published in peer-reviewed journals and presented in local and national symposiums. Currently, there are several projects that will benefit directly from the service provided by this microscope-based technology. Therefore, the aims of the Optical Imaging Facility are:1. To provide technical support for the time and spatial resolution imaging systems.2. To integrate an immunocytochemistry laboratory into the Optical Imaging Facility.3. To disseminate in our academic community the capabilities of our facility.4. To work on the submission of a grant for a confocal microscope.The continued support of the Optical Imaging Facility will constitute an asset for the Cell and Molecular Biology Center since it will contribute to enhance the productivity of its current participants and will constitute an attractive research resource for potential new investigators/ collaborators. The immunocytochemisty capabilities at the Optical Imaging Facility will complement our existing imaging platform that is configured for the rapid acquisition and analysis of fluorescently tagged reporters of intracellular calcium.