DNA SEQUENCING AND ANALYSIS CORE FACILITY

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 DNA分析，特别是大量DNA基因组信息的应用，是现代生物医学研究的一个重要方面。为了促进应用最先进的技术来研究与得克萨斯/墨西哥边境地区有关的问题，这项提议将支持开发DNA分析核心设施。该设施将把现有的共享设备和研究人员拥有的设备结合在一起，包括DNA测序、基因分析、实时聚合酶链式反应和专门的电泳能力，形成一个管理的、研究人员可访问的核心设施。除了提供工作人员支持外，这项提议还将通过增加一个定制的微阵列设施来加强现有的仪器基础设施。这种新的定制排列能力是支持现有毒物应激、传染病分子流行病学和代谢紊乱研究项目发展的基本要素。虽然该设施最初将在现有空间建立，但在这些新实验室建成后，它将搬到埃尔帕索得克萨斯大学新的生物医学大楼的扩大宿舍。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. DNA analysis, particularly the application of the deluge of DNA genomic information, is an essential aspect of modern day biomedical research. In order to facilitate the application of state of the art techniques to research problems relevant to the Texas/Mexico border region, this proposal will support the development of a DNA analysis core facility. This facility will combine existing shared and investigator owned equipment, including DNA sequencing, Genotype analysis, real time PCR, and specialized electrophoresis capability into a managed, researcher accessible core facility. In addition to providing staff support, this proposal will augment existing instrument infrastructure by the addition of a custom microarraying facility. This new custom arraying capability is an essential element in support of development of existing research projects in toxicant stress, the molecular epidemiology of infectious diseases, and metabolic disorders. While the facility will initially be setup in existing space, it will move to expanded quarters in the new Biomedical Sciences Building at the University of Texas in El Paso upon completion of those new laboratories.