SULFUR OXIDATION IN C ELEGANS BY OXIDATIVE STRESS AS COMPARED TO THE AGING PROC

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 线虫作为一种模式生物，由于其寿命短，交配容易控制，非常适合于衰老研究。雅各布斯实验室在线虫和衰老方面进行了广泛的研究，最近的工作是研究氧化应激的作用。他们和其他人一起开发了OxICAT系统，用于定位氧化还原活性硫醇蛋白质组。他们的结论表明，尽管氧化应激和衰老都会导致可逆氧化的半胱氨酸残基水平相当，但目标蛋白是不同的。然而，他们目前的方法只能将半胱氨酸氧化成二硫化物，而不能将半胱氨酸氧化成硫酸或磺酸。与雅各布斯实验室合作，我们建议使用硫XANES来绘制总硫氧化状态以及由于老化过程而发生的不可逆硫氧化量，并与氧化应激相比较。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. C. elegans as a model organism is well suited for studies in aging due to its short life span and easily controlled mating. The Jakobs lab has worked extensively on C. elegans and aging, most recently working on the role of oxidative stress. They, with other have developed the OxICAT system for mapping the redox active thiol proteome. Their conclusions show that while oxidative stress and aging both lead to comparable levels of reversibly oxidized cysteine residues, target proteins are different. However, their current methods can only map cysteines oxidized to disulphides and not cysteines oxidized to sulfunic or sulfonic acids. In conjunction with the Jakobs lab, we propose to use Sulfur XANES to map the total sulfur oxidation state and the amount of irreversible sulfur oxidation that happens as a result of the aging process compared to that of oxidative stress.