DIGITAL IMAGING OF FIREFLY LUCIFERASE ACTIVITY IN MOUSE EMBRYONIC STEM CELLS
DIGITAL IMAGING OF FIREFLY LUCIFERASE ACTIVITY IN MOUSE EMBRYONIC STEM CELLS
批准号:
7956509
负责人:
Ken W.Y. Cho
金额:
$0.53万
依托单位国家:
美国
项目类别:
财政年份:
2009
资助国家:
美国
项目状态:
已结题
起止时间:
2009-08-01 至 2010-07-31
关键词:
Computer Retrieval of Information on Scientific Projects DatabaseES Cell LineFirefly LuciferasesFluorescenceFundingGrantInstitutionLaboratoriesLight ExerciseLuciferasesMusPlasmidsReporterResearchResearch PersonnelResourcesSourceStem cellsUnited States National Institutes of Healthcharge coupled device cameradigital imagingembryonic stem cell
中文摘要
这个子项目是许多研究子项目中的一个
由NIH/NCRR资助的中心赠款提供的资源。子项目和
研究者(PI)可能从另一个NIH来源获得了主要资金,
因此可以在其他CRISP条目中表示。所列机构为
研究中心,而研究中心不一定是研究者所在的机构。
我们将CMV-荧光素酶质粒转染到小鼠胚胎干细胞中,然后通过CCD相机捕捉光活性来观察荧光素酶活性。 我们也正在产生一个稳定的BMP响应荧光素酶报告小鼠胚胎干细胞系,并查看我们的干细胞的荧光素酶活性。
英文摘要
This subproject is one of many research subprojects utilizing the
resources provided by a Center grant funded by NIH/NCRR. The subproject and
investigator (PI) may have received primary funding from another NIH source,
and thus could be represented in other CRISP entries. The institution listed is
for the Center, which is not necessarily the institution for the investigator.
We are transfecting CMV-Luciferase plasmid into mouse embryonic stem cells and then viewing luciferase activity via a CCD camera to capture light activity. We are also generating a stable BMP responsive luciferase reporter mouse embryonic stem cell line and look at luciferase activity of our stem cells.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
Spatiotemporal mapping of enhancer activity in developing frog embryos
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批准号:10511083
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项目类别:
-
资助金额:$22.82万
-
财政年份:2022
-
负责人:Ken W.Y. Cho
-
依托单位:
Spatiotemporal mapping of enhancer activity in developing frog embryos
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批准号:10686937
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项目类别:
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资助金额:$18.85万
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财政年份:2022
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负责人:Ken W.Y. Cho
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依托单位:
Maternal transcription factors shaping early embryonic chromatin landscape
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批准号:10353368
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项目类别:
-
资助金额:$40.27万
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财政年份:2021
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负责人:Ken W.Y. Cho
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依托单位:
Maternal transcription factors shaping early embryonic chromatin landscape
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批准号:10570971
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项目类别:
-
资助金额:$41.05万
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财政年份:2021
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负责人:Ken W.Y. Cho
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依托单位:
Maternal transcription factors shaping early embryonic chromatin landscape
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批准号:10389644
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项目类别:
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资助金额:$14.9万
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财政年份:2021
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负责人:Ken W.Y. Cho
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依托单位:
Assessment of the phasor Fluorescence Lifetime Imaging Microscopy (FLIM) Approach in an animal model
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批准号:9396700
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项目类别:
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资助金额:$22.19万
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财政年份:2017
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负责人:Ken W.Y. Cho
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依托单位:
Deciphering the gene regulatory network controlling vertebrate endodermal fates
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批准号:9256494
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项目类别:
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资助金额:$56.04万
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财政年份:2013
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负责人:Ken W.Y. Cho
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依托单位:
Deciphering the gene regulatory network controlling vertebrate endodermal fates
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批准号:8858659
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项目类别:
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资助金额:$57.59万
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财政年份:2013
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负责人:Ken W.Y. Cho
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依托单位:
Deciphering the gene regulatory network controlling vertebrate endodermal fates
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批准号:8692986
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项目类别:
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资助金额:$58.37万
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财政年份:2013
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负责人:Ken W.Y. Cho
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依托单位:
Deciphering the gene regulatory network controlling vertebrate endodermal fates
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批准号:9054884
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项目类别:
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资助金额:$57.19万
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财政年份:2013
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负责人:Ken W.Y. Cho
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依托单位:
Deciphering the gene regulatory network controlling vertebrate endodermal fates
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批准号:8561007
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项目类别:
-
资助金额:$62.8万
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财政年份:2013
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负责人:Ken W.Y. Cho
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依托单位:
Transcriptome Profiling and Targeted Genic Improvement of the X. tropicalis Genom
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批准号:8154033
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项目类别:
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资助金额:$56.45万
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财政年份:2011
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负责人:Ken W.Y. Cho
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依托单位:
Transcriptome Profiling and Targeted Genic Improvement of the X. tropicalis Genom
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批准号:8460905
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项目类别:
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资助金额:$48.52万
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财政年份:2011
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负责人:Ken W.Y. Cho
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依托单位:
Transcriptome Profiling and Targeted Genic Improvement of the X. tropicalis Genom
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批准号:8304922
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项目类别:
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资助金额:$50.87万
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财政年份:2011
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负责人:Ken W.Y. Cho
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依托单位:
Transcriptome Profiling and Targeted Genic Improvement of the X. tropicalis Genom
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批准号:8653581
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项目类别:
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资助金额:$48.86万
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财政年份:2011
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负责人:Ken W.Y. Cho
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依托单位:
BMP signaling in vertebrate development
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批准号:8442378
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项目类别:
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资助金额:$26.98万
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财政年份:2009
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负责人:Ken W.Y. Cho
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依托单位:
BMP signaling in vertebrate development
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批准号:7779396
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项目类别:
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资助金额:$30.21万
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财政年份:2009
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负责人:Ken W.Y. Cho
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依托单位:
BMP signaling in vertebrate development
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批准号:8063083
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项目类别:
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资助金额:$28.83万
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财政年份:2009
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负责人:Ken W.Y. Cho
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依托单位:
Regulation of call adhesion in Xenopus
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批准号:7924292
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项目类别:
-
资助金额:$9.42万
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财政年份:2009
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负责人:Ken W.Y. Cho
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依托单位:
BMP signaling in vertebrate development
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批准号:8242087
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项目类别:
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资助金额:$28.65万
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财政年份:2009
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负责人:Ken W.Y. Cho
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依托单位:
海外基金