HISTONE METHYLATION AND EPIGENETIC CONTROL OF THE GENOME

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 组蛋白的残基特异性甲基化导致不同的表观遗传状态。但是，组蛋白甲基转移酶(向组蛋白中添加甲基的酶)和组蛋白去甲基酶(从组蛋白中去掉甲基的酶)是如何针对特定的染色体区域的还不是很清楚。更重要的是，除了几个众所周知的例子外，细胞机制如何识别这些修改还不清楚。我们将从分裂酵母中提纯组蛋白甲基转移酶和去甲基酶复合体，以鉴定调控组蛋白甲基化的新蛋白。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Residue specific methylation of histones results in diverse epigenetic states. But how histone methyltransferases (enzymes that add methyl group to histones) and histone demethylases (enzymes that remove methyl group from histones) are targeted to specific chromosome domains are not well understood. More importantly, apart from several well-known examples, how the cellular machinery recognizes these modifications is not clear. We will purify histone methyltransferase and demethylases complexes from fission yeast to identify novel proteins that regulate histone methylation.