DESIGN OF SELF-ASSEMBLED 3D DNA CRYSTALS USING 6HB

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 结构DNA纳米技术使用不寻常的DNA基序来构建目标形状和周期性排列。我们希望这些系统可以应用于几个实际目的：这项研究的关键动机目标是空间周期网络是晶体。此外，这项研究最严峻的挑战是构建高阶3D晶体。如果我们能在纳米尺度上建造棒状水晶笼子，它们就可以用来定向其他生物大分子在这些笼子里作为客体，从而使它们的3D结构适合于衍射分析。蛋白质的球状形状不利于形成有序晶体所必需的堆积排列，由于DNA和蛋白质之间的特殊相互作用，DNA 3D晶体是形成周期性晶体笼子的极佳候选者，可以容纳蛋白质以进行X射线衍射研究；同样的晶体阵列可以用于纳米级的分子电子器件的定位和定向。 许多使用不同寻常的DNA图案的设计已经被自我组装成3D晶体。虽然我们已经成功地建立了2D阵列，但2D排列成功的结构标准通常是基于分辨率极限为3-10 nm的AFM观察。相比之下，x射线衍射的成功工作的目标是2？？左右。然而，我们的初晶的衍射图被限制在10？？六-DNA螺旋束正是本研究使用的模体。在这个模体中，如果你往下看DNA的螺旋轴，你会发现六个DNA螺旋结合成一个六边形的排列。我们使用了热控和蒸汽扩散的方法。我们已经用前一种方法成功地制作了二维阵列。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Structural DNA nanotechnology uses unusual DNA motifs to build target shapes and periodic arrangements. We expect these systems can be applied to several practical ends: The key motivating goal for this research is that spatially periodic networks are crystals. Also the Steepest challenge of the research is the construction of 3D crystals with high order. If we can build stick-figure crystalline cages in the nanometer scale, they could be used to orient other biological macromolecules as guests inside those cages, thereby rendering their 3D structure amenable to diffraction analysis. The globular shapes of proteins are not conducive to the packing alignment that is essential to form a well-ordered crystal, due to specific interactions between DNA and proteins, DNA 3D crystalline crystal is an excellent candidate for forming periodic crystalline cages that can host proteins in order for the X-ray diffraction studies; Similarly, the same crystalline arrays could be used to position and orient components of molecular electronic devices with nanometer-scale precision. A number of designs using unusual DNA motifs have been self-assembled to yield 3D crystals. Although we have been successfully build 2D arrays, the structural criteria of success with 2D arrangements are based typically on AFM observation with resolution limit of 3-10 nm. By contrast the goal for successful work of x-ray diffraction is around 2 ¿¿¿ . However the diffraction patterns of our preliminary crystals have been limited to 10 ¿¿¿ . Six-DNA helix-Bundle is the exact motif I¿¿¿¿¿"m using for this research. In this motif six DNA helices associate to form a hexagon arrangement if you look down the helical axis of DNA. We use thermo-control and vapour-diffusion methods. We have been successfully made 2D arrays by using the former method.