HUMAN REV1 TRANSLESION DNA POLYMERASE

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 Rev1是DNA复制机制的关键部分，存在于所有真核生物中。跨损伤合成是指识别并复制基因组中持续发生的错误，使高保真聚合酶能够完成复制基因组的功能的过程。Y家族的DNA聚合酶包含各种酶，专用于不同类型的DNA损伤和旁路过程的不同阶段。在Rev1的情况下，该酶高度专一性地掺入胞苷，而不是具有氮加合物的G。我们实验室以前解决的酵母酶结构表明了一种通过蛋白质模板指定传入核苷酸的独特方法。人们希望这项工作将深入了解人类酶的机制，并确定与酵母蛋白不同功能相关的新结构域。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Rev1 is a key part of the DNA replication machinery and is found in all eukaryotes. Translesion synthesis is the process by which errors that continually occur within the genome are identified and replicated through to enable the high fidelity polymerases to complete their function of replicating the genome. The Y family of DNA polymerases encompasses a variety of enzymes specific for different types of DNA lesions and different stages in the bypass process. In the case of Rev1 the enzyme is highly specific for incorporation of cytidine opposite a G that has an N2 adduct. A previous structure solved in our lab of the yeast enzyme indicated a unique method of specifying the incoming nucleotide via a protein template. It is hoped that this work will give insights into the mechanism seen in the human enzyme and identify novel domains associated with different functions to the yeast protein.