ANALYZING ELECTRON TOMOGRAPHY DATA WITH IMOD AND CHIMERA

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 该项目旨在促进结合使用IMOD和Chimera软件包分析细胞和病毒的电子断层扫描(ET)数据。IMOD是用于ET地图手工分割的主要程序，主要基于2维数据切片的可视化，而Chimera主要用于3维可视化。IMOD还从实验中获得的2维倾斜序列计算层析重建。IMOD和Chimera的互补能力经常被研究人员在分析ET数据时使用。IMOD是由博尔德实验室的David Mastronarde开发的，用于细胞的三维电子显微镜，这是由Andreas Hoenger指导的NCRR生物医学技术研究资源。这一合作项目对IMOD和Chimera软件进行了改进，以简化方案之间分段和地图数据的交换。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This project aims to facilitate the combined use of the IMOD and Chimera software packages in analysis of electron tomography (ET) data of cells and viruses. IMOD is the main program used for hand-segmentation of ET maps and is based largely on visualization of 2-dimensional data slices, while Chimera is primarily used for 3-dimensional visualization. IMOD also computes tomographic reconstructions from the 2-dimensional tilt series obtained in experiments. Complementary capabilities of IMOD and Chimera are frequently used by researchers analyzing ET data. IMOD is developed by David Mastronarde at the Boulder Laboratory for 3-D Electron Microscopy of Cells which is an NCRR Biomedical Technology Research Resource directed by Andreas Hoenger. This collaborative project undertakes improvements in IMOD and Chimera software to simplify the exchange of segmentation and map data between the programs.