SIMULATION OF RECEPTOR-LIGAND-MEDIATED CELLULAR ADHESION IN A LINEAR SHEAR FIEL

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 在循环系统中发生的许多生理过程中，存在血流动力学流动时的细胞黏附起着重要作用。这些黏附相互作用受到截然不同的长度尺度上的过程的影响：(1)使细胞变形的流体力是在细胞直径的长度尺度上(~10m)，而(2)介导细胞黏附的受体-配体键的范围是~100 nm。作用在这些不同长度尺度上的力的耦合以及它们对细胞变形的影响使得细胞黏附的模拟需要大量的计算。我们用浸没边界方法模拟了多形核白细胞在线性剪切场中的同型聚集，并对受体-配体相互作用进行了随机描述。这些细胞被模拟成包裹着牛顿流体的球形Neo-Hookean膜。这项研究将阐明流体剪切和细胞变形性对白细胞聚集效率的影响。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Cell adhesion in the presence of hemodynamic flow plays a fundamental role in many of the physiological processes occurring in the circulatory system. These adhesive interactions are influenced by processes taking place at drastically different length scales: (1) Fluid forces which tend to deform the cells are on the length scale of the cell diameter (~10 ?m) whereas (2) the range of receptor-ligand bonds mediating cell adhesion is ~ 100 nm. The coupling of forces acting at these disparate length scales as well as their effects on cell deformation makes the simulation of cell adhesion computationally intensive. We simulate the homotypic aggregation of polymorphonuclear leukocytes in a linear shear field using the immersed boundary method with a stochastic description of receptor-ligand interactions. The cells have been modeled as spherical Neo-Hookean membranes enclosing a Newtonian fluid. This study will shed light on the effects of fluid shear and cell deformability on the efficiency of leukocyte aggregation.