High-Throughput Cell Mechanical Property Testing for Label-Free Assaying
用于无标记测定的高通量细胞机械特性测试
基本信息
- 批准号:8305759
- 负责人:
- 金额:$ 31.73万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2009
- 资助国家:美国
- 起止时间:2009-08-15 至 2014-07-31
- 项目状态:已结题
- 来源:
- 关键词:Biological AssayCell CountCell SeparationCell SurvivalCellsChemicalsCollaborationsCouplingDataDetectionDevelopmentDevice DesignsDevicesErythrocytesHealthImageIndividualInfectionInvestigationLabelLaboratoriesLaboratory ResearchLasersLeadLifeMalariaMeasurementMeasuresMechanicsMethodsMicrofluidicsModelingMolecularMonitorNational Institute of Allergy and Infectious DiseaseOptical MethodsOpticsParasitesPerformancePhasePhysiologyPropertyReagentResearchRheologySchemeSignal TransductionSourceSpeedStretchingSuspension substanceSuspensionsSystemTechniquesTechnologyTestingTimeUnited States National Institutes of HealthWorkbasecell typecosthigh throughput analysishigh throughput screeningimprovedinstrumentinstrumentationoptical trapsphysical propertyresponsesensorvector
项目摘要
Project Summary: We introduce a unique microfluidic-based approach for the high-throughput
non-destructive assaying of cells without the need for specific labels or reagents. Based on
measurement of both static and dynamic cell mechanical properties using applied optical forces,
we will apply this technique (known as "optical stretching") in a high-speed high-throughput
manner. To date, optical stretching has been used only on small cell numbers; however, high-
intensity, microscale laser sources and the integration of these within dynamic microfluidic
systems has enabled our proposed approach. In this, fully integrated optical-based sensors and
mechanical stretchers will be used to identify and, upon demand, isolate single cells. Once
identified, such targeted cells can then be transported on-chip to culture chambers within the
device or for dispensing into standard bio-laboratory instrumentation for off-chip analysis.
Though there is broad need, our proposed technology will be tested and developed using
malaria parasite infected red blood cells as the target cell. This work will be done in
collaboration with the Laboratory of Malaria and Vector Research at the NIAID. Our aims
include: Aim 1: Mechanical Property Detection and Interpretation. We will employ optical
manipulation methods integrated within microfluidic systems for label-free, non-destructive cell
mechanical property measurement. Modeling approaches will be developed for both
interpretation of applied force/deformation experimental data and for device design. Here,
malaria-infected red blood cells will provide a good model target since cell stiffness changes
dramatically during parasite development. Demonstrating greatly simplified device designs and
associated ease-of-use, we will install an instrument in an active NIH laboratory. Aim 2: Optical
Manipulation for Cell Identification and Isolation. We will integrate optical methods within
microfluidic systems for single cell detection and manipulation. Here, methods for both on-chip
cell isolation and off-chip isolation will be developed and used to improve our installed NIH
protototype. Aim 3: High Throughput Mechanical Testing. To achieve high-throughputs,
modified microfluidic and faster detection techniques will be required. In this phase, the coupling
of hydrodynamic and optical forces will be explored to improve device performance. In addition,
time-varying optical forces will be employed to identify optimal signal response and dynamic
physical properties.
项目概述:我们介绍了一种独特的基于微流体的高通量方法,
不需要特定标记或试剂的细胞的非破坏性测定。基于
使用施加的光学力测量静态和动态细胞机械性能,
我们将把这种技术(称为“光学拉伸”)应用于高速高通量
方式迄今为止,光学拉伸仅用于小细胞数;然而,高-
强度、微尺度激光源以及这些在动态微流体中的集成
系统实现了我们提出的方法。在这方面,完全集成的光学传感器和
将使用机械拉伸器来鉴定并根据需要分离单细胞。一旦
鉴定后,然后可以将这样的靶细胞在芯片上运输到培养室内的培养室。
设备或用于分配到标准生物实验室仪器中以进行芯片外分析。
虽然有广泛的需求,我们提出的技术将进行测试和开发,
疟疾寄生虫感染的红细胞作为靶细胞。这项工作将在
与NIAID的疟疾和病媒研究实验室合作。我们的目标
包括:目标1:机械性能检测和解释。我们将采用光学
集成在微流控系统内的无标记、非破坏性细胞的操作方法
机械性能测试将为这两个方面制定建模方法
解释所施加的力/变形实验数据和用于装置设计。在这里,
感染疟疾的红细胞将提供一个很好的模型目标,因为细胞硬度的变化
在寄生虫发育过程中发生了巨大变化。展示了大大简化的设备设计,
相关的易用性,我们将在一个活跃的NIH实验室安装一台仪器。目标2:光学
用于细胞鉴定和分离的操作。我们将把光学方法整合到
用于单细胞检测和操作的微流体系统。在这里,用于片上
细胞隔离和芯片外隔离将被开发和用于改善我们安装的NIH
原型目标3:高吞吐量机械测试。为了实现高吞吐量,
将需要改进的微流体和更快的检测技术。在这个阶段,耦合
将探索流体动力学和光学力的作用,以提高器件性能。此外,本发明还提供了一种方法,
时变光学力将用于识别最佳信号响应和动态
物理性质
项目成果
期刊论文数量(10)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Cell elongation via intrinsic antipodal stretching forces.
通过内在的反足拉伸力使细胞伸长。
- DOI:10.1103/physreve.86.061901
- 发表时间:2012
- 期刊:
- 影响因子:0
- 作者:Sawetzki,T;Eggleton,CD;Marr,DWM
- 通讯作者:Marr,DWM
FACS-style detection for real-time cell viscoelastic cytometry.
用于实时细胞粘弹性细胞计数的 FACS 式检测。
- DOI:10.1039/c5ra24097b
- 发表时间:2015
- 期刊:
- 影响因子:3.9
- 作者:Kasukurti,A;Eggleton,CD;Desai,SA;Marr,DWM
- 通讯作者:Marr,DWM
A simple microfluidic dispenser for single-microparticle and cell samples.
用于单微粒和细胞样品的简单微流体分配器。
- DOI:10.1039/c4lc00863d
- 发表时间:2014-12-21
- 期刊:
- 影响因子:6.1
- 作者:Kasukurti A;Eggleton CD;Desai SA;Disharoon DI;Marr DW
- 通讯作者:Marr DW
Dynamic ray tracing for modeling optical cell manipulation.
- DOI:10.1364/oe.18.016702
- 发表时间:2010-08-02
- 期刊:
- 影响因子:3.8
- 作者:Sraj I;Szatmary AC;Marr DW;Eggleton CD
- 通讯作者:Eggleton CD
High-throughput linear optical stretcher for mechanical characterization of blood cells.
- DOI:10.1002/cyto.a.22794
- 发表时间:2016-04
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
CHARLES Dionisio EGGLETON其他文献
CHARLES Dionisio EGGLETON的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('CHARLES Dionisio EGGLETON', 18)}}的其他基金
SIMULATION OF RECEPTOR-LIGAND-MEDIATED CELLULAR ADHESION IN A LINEAR SHEAR FIEL
线性剪切场中受体-配体介导的细胞粘附的模拟
- 批准号:
8171899 - 财政年份:2010
- 资助金额:
$ 31.73万 - 项目类别:
SIMULATION OF RECEPTOR-LIGAND-MEDIATED CELLULAR ADHESION IN A LINEAR SHEAR FIEL
线性剪切场中受体-配体介导的细胞粘附的模拟
- 批准号:
7956360 - 财政年份:2009
- 资助金额:
$ 31.73万 - 项目类别:
High-Throughput Cell Mechanical Property Testing for Label-Free Assaying
用于无标记测定的高通量细胞机械特性测试
- 批准号:
7736282 - 财政年份:2009
- 资助金额:
$ 31.73万 - 项目类别:
High-Throughput Cell Mechanical Property Testing for Label-Free Assaying
用于无标记测定的高通量细胞机械特性测试
- 批准号:
7916769 - 财政年份:2009
- 资助金额:
$ 31.73万 - 项目类别:
High-Throughput Cell Mechanical Property Testing for Label-Free Assaying
用于无标记测定的高通量细胞机械特性测试
- 批准号:
8103049 - 财政年份:2009
- 资助金额:
$ 31.73万 - 项目类别:
Computational model of cellular adhesion in bulk flows
散装流中细胞粘附的计算模型
- 批准号:
6863858 - 财政年份:2005
- 资助金额:
$ 31.73万 - 项目类别:
Computational model of cellular adhesion in bulk flows
散装流中细胞粘附的计算模型
- 批准号:
7017762 - 财政年份:2005
- 资助金额:
$ 31.73万 - 项目类别:
Computational model of cellular adhesion in bulk flows
散装流中细胞粘附的计算模型
- 批准号:
7561012 - 财政年份:2005
- 资助金额:
$ 31.73万 - 项目类别:
Computational model of cellular adhesion in bulk flows
整体流中细胞粘附的计算模型
- 批准号:
7343186 - 财政年份:2005
- 资助金额:
$ 31.73万 - 项目类别:
Computational model of cellular adhesion in bulk flows
散装流中细胞粘附的计算模型
- 批准号:
7216394 - 财政年份:2005
- 资助金额:
$ 31.73万 - 项目类别:
相似海外基金
Determination of the optimum peak cell count for each yeast strain.
确定每种酵母菌株的最佳峰值细胞计数。
- 批准号:
494444-2016 - 财政年份:2016
- 资助金额:
$ 31.73万 - 项目类别:
Experience Awards (previously Industrial Undergraduate Student Research Awards)
Point-of-care blood cell count through micro-ELISA
通过微量 ELISA 进行即时血细胞计数
- 批准号:
131948 - 财政年份:2015
- 资助金额:
$ 31.73万 - 项目类别:
Feasibility Studies
THE COMPARISON OF PLACENTAL AND UMBILICAL NUCLEATED RED BLOOD CELL COUNT
胎盘和脐带有核红细胞计数的比较
- 批准号:
7376109 - 财政年份:2005
- 资助金额:
$ 31.73万 - 项目类别:
THE COMPARISON OF PLACENTAL AND UMBILICAL NUCLEATED RED BLOOD CELL COUNT
胎盘和脐带有核红细胞计数的比较
- 批准号:
7206410 - 财政年份:2004
- 资助金额:
$ 31.73万 - 项目类别:
A Simple Point of Care White Blood Cell Count Test
简单的护理点白细胞计数测试
- 批准号:
6830358 - 财政年份:2001
- 资助金额:
$ 31.73万 - 项目类别:
A Simple Point Of Care White Blood Cell Count Test
简单的护理点白细胞计数测试
- 批准号:
6403018 - 财政年份:2001
- 资助金额:
$ 31.73万 - 项目类别:
A Simple Point of Care White Blood Cell Count Test
简单的护理点白细胞计数测试
- 批准号:
6943868 - 财政年份:2001
- 资助金额:
$ 31.73万 - 项目类别: