STRUCTURE OF THE PHAGE MU TRANSPOSOSOME ASSEMBLED ON SUPERCOILED DNA

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 转座因子是基因组进化的驱动力。噬菌体Mu是已知研究最充分和最有效的转座因子。转座的化学反应发生在一个稳定的核蛋白复合物中，称为“转座体”，它是由三个DNA位点-Mu的左（L）和右（R）端和增强子元件（E）-之间的桥接相互作用建立的，由转座酶蛋白MuA的六个亚基和E的二聚体介导。coli蛋白HU。DNA在转座体内遵循一条明确的路径，捕获五个超螺旋节点。DNA和蛋白质组分的特殊排列为转座体提供了非凡的稳定性，并调节转座的频率、精确度、方向性和机制。因此，转座体的结构是理解所有这些属性的关键。并最终解释了转座因子在整个生物世界中失控的遗传成功。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Transposable elements are the drivers of genome evolution. Phage Mu is the most well-studied and most efficient transposable element known. The chemistry of transposition occurs within a stable nucleoprotein complex called the 'transpososome' which is built bridging interactions among three DNA sites - the left (L) and right (R) ends of Mu and an enhancer element (E) - mediated by the six subunits of the transposase protein MuA and a dimer of the E. coli protein HU. The DNA follows a well-defined path within the transpososome, trapping five supercoil nodes. The particular arrangement of DNA and protein components lends extraordinary stability to the transpososome and regulates the frequency, precision, directionality and mechanism of transposition. The structure of the transpososome, therefore, holds the key to understanding all of these attributes. and ultimately to explaining the runaway genetic success of transposable elements throughout the biological world.