METABOLOMICS OF RUBUS TRICHOMES:EXPLORATIONOF POTENTIAL SIGNALS W/CELL SIGNALING

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者的研究机构。 控制细胞增殖的细胞间通讯途径对癌症生物学和植物生长都很重要。 为了研究细胞增殖，我们选择了植物刺，因为它们很容易观察到，并在植物的整个生命周期中生长。 皮刺的结构也很简单，由表皮组织和皮质组织的生长物组成。 因此，由于它们的结构简单，棘突发育提供了一个理想的系统来研究细胞如何通信以控制解剖结构的生长、增殖和形态分化  所有发展途径的基本方面。 为了研究这个器官，我们选择了黑莓和乌藨子，它们都有刺和无刺的品种。 我在2006年7月发起了一个项目，探索刺痛的发展。 最初的研究表明，刺要么直接从头状茎毛状体（树莓）发展，要么可能提供一个信号，允许底层皮层细胞（黑莓）增殖。 为了深入了解这些过程的分子控制，我们已经启动了相关基因候选人的基因组学和功能研究。 然而，研究潜在的代谢物并将其与我们正在研究的候选基因联系起来也是至关重要的。 一种这样的代谢候选物是没食子酸，其抑制人类前列腺癌细胞中的细胞周期并且还防止血管生成;然而，我们认为它在植物组织中可能具有相反的作用，并且允许植物细胞从细胞周期中去除有丝分裂并进入内复制，这是毛状体（和潜在的刺痒发育）的关键过程。 我们建议测试的假设，即没食子酸确实存在于头状茎毛状体，因此在植物与动物细胞信号和生长中起着二分法的作用。 为了完成这项研究，我将与UAMS（分子生物学）的玛丽周博士和UALR（代谢组学）的斯蒂芬格雷斯博士合作。 这种合作将增加我们各自领域之间未来的合作机会，从而通过INBRE计划为本科生的研究经验增加许多新的机会。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The cell-to-cell communication pathways that control cell proliferation are important to both cancer biology and plant growth. In order to study cell proliferation we have selected plant prickles because they are easily observed and grow throughout the life of the plant. Prickles are also structurally simple, comprised of outgrowths of epidermal and sometimes cortical tissues. Thus, because of their structural simplicity, prickle development, provides an ideal system to study how cells communicate to control growth, proliferation, and morphological differentiation of an anatomical structure  a fundamental aspect of all developmental pathways. To study this organ we have selected blackberries and raspberries, which have both prickled and prickleless varieties. I initiated a project in July 2006 to explore prickle development. Initial studies suggest that prickles either develop directly from Capitate-Stalked trichomes (raspberry) or may provide a signal that allows proliferation of the underlying cortical cells (blackberry). To gain insight into the molecular control of these processes, we have initiated genomics and functional studies of gene candidates involved. However, it will be critical to also look at potential metabolites and relate those back to the gene candidates we are studying. One such metabolic candidate is gallic acid that inhibits the cell cycle in human prostate cancer cells and also prevents angiogenesis; however, we believe that it may have an opposite effect in plant tissues and allow the plant cells to remove mitosis from the cell cycle and enter endoreplication, a process critical for trichome (and potentially prickle development). We are proposing to test the hypothesis that gallic acid is indeed present in Capitate-Stalked trichomes and thus plays a dichotomous role in plant versus animal cell signaling and growth. To complete this research I will be working with Drs. Marie Chow of UAMS (molecular biology) and Dr. Stephen Grace of UALR (metabolomics). This collaboration will increase future opportunities for collaboration between our respective fields and thus increase many new opportunities for undergraduate research experiences through the INBRE program.