Flow Cytometry

阿拉巴马大学艾滋病研究中心(CFAR)流式细胞术核心的主要目标是 提供最先进的流式细胞仪服务，支持艾滋病研究项目。流式细胞术是一种独特的 分析颗粒荧光性质的强大技术，可轻松应用于测定 细胞的表型和功能，以及用于通过分类分离确定的细胞群体。近年来， 随着新的解剖方法的出现，流式细胞仪分析在艾滋病毒研究中的应用变得越来越突出 艾滋病毒与免疫系统细胞之间的相互作用已经发展；基于荧光的记者 系统已被利用来监控病毒复制、重新激活和重组；以及复杂的 仪器技术的进步为高速多色分析提供了新的平台 细胞分类。因此，CFAR流式细胞仪核心是促进和促进的重要和多才多艺的资源 艾滋病研究。流式细胞仪核心的使命是通过提供 在组织良好、集中化、能够处理潜在传染性的设施内提供必要的工具 材料。它提供培训机会和咨询，也是促进互动的渠道。 研究人员和具有不同兴趣和专业知识的实验室人员之间的联系。在之前的资助期间 在此期间，CFAR流式细胞仪核心进行了重大设备升级，安装了BDFACSAria 细胞分选仪以及BD-LSR-II分析型流式细胞仪。流式细胞仪核心也改进了它的 提高知名度和可及性，以及加强教育和培训举措。作为这些努力的结果， 自2002年以来，流式细胞仪核心的使用量增加了三倍多。流式细胞仪核心有 支持来自69个主要调查实验室的184名不同个人的研究活动，并已 在病毒发病机制、抗病毒研究、细胞学领域的基础、临床和翻译倡议方面做出了贡献 免疫力和疫苗设计。该核心支持了88项国立卫生研究院赠款的活动，并为172 出版物流式细胞仪核心的目标是现在通过进一步增强 服务以及教育和培训活动，使用户能够充分利用先进的 可用的工具。这些努力包括，根据需求评估分析，安装一个 第三代流式细胞仪和BioPlex流式细胞仪用于生物分子(如细胞因子)的多重分析 2008年初。我们还致力于通过与用户合作、促进协作和CrossCore来促进创新 鼓励前沿艾滋病相关研究活动的伙伴关系。

The major objective of the University of Alabama (DAB) Center for AIDS Research (CFAR) Flow Cytometry Core is to provide state-of-the-art flow cytometry services to support AIDS research programs. Flow cytometry is a unique and powerful technology for analyzing the fluorescent properties of particles and can be readily applied to determining the phenotype and function of cells, as well as utilized for isolating defined cell populations by sorting. In recent years the use of flow cytometric analyses in HIV research has become ever more prominent as newer approaches for dissecting the interactions between HIV and cells of the immune system have been developed; fluorescent based reporter systems have been harnessed to monitor virus replication, reactivation, and recombination; and sophisticated technological advances in instrumentation have provided new platforms for high-speed polychromatic analyses and cell sorting. The CFAR flow cytometry core is, therefore, a vital and versatile resource for promoting and facilitating AIDS research. The flow cytometry core's mission is to maximize the benefits of this technology by providing the necessary instrumentation within a well organized, centralized, facility that is capable of handling potentially infectious material. It provides training opportunities and consultations, and it also serves as a conduit for fostering interactions between investigators and laboratory personnel with diverse interests and expertise. During the previous funding period the CFAR flow cytometry core has undergone a significant equipment upgrade, with the installation of a BDFACSAria cell sorter as well as a BD-LSR-II analytical flow cytometer. The flow cytometry core has also improved its visibility and accessibility as well as enhanced education and training initiatives. As a result of these endeavors the usage of the flow cytometry core has increased more than three-fold since 2002. The flow cytometry core has supported the research activities of 184 different individuals from 69 Principle Investigators laboratories and has contributed to basic, clinical, and translational initiatives in the areas of viral pathogenesis, anti-viral research, cellular immunity, and vaccine design. The core has supported the activities of 88 NIH grants and contributed to 172 publications The aims of the flow cytometry core are to now build upon our progress-to-date by further enhancing services, and education and training activities so that users are empowered to take full advantage of the sophisticated instrumentation that is available. These efforts include, based on a needs assessment analysis, the installation of a third flow cytometer and a BioPlex flow cytometer for multiplexed analysis of biomolecules (e.g. cytokines) in the beginning of 2008. We also aim to promote innovation by working with users, and fostering collaborations and crosscore partnerships that encourage cutting-edge AIDS related research activities.