A Combined 600 MHz NMR Console for Studies of Cell Extracts and Biological Solids

描述(由申请人提供)：本申请要求最先进的组件，以1)在加州大学旧金山分校建立生物固态核磁共振；以及2)显著加强目前专注于研究人类疾病的代谢途径和生物标记物的努力。所需要的仪器是液体和固态600 MHz核磁共振仪控制台和附件，它允许：1)高分辨率三共振魔角旋转实验，以执行哺乳动物和真菌Prion蛋白纤维的构象分析；2)高通量、自动化的多核检测，以建立与核磁共振可检测的生物标记物的关联；以及3)开发新型的超灵敏核磁共振技术，利用动态核极化监测配体与大分子的结合。这些领域中的每一个都得到了加州大学NIH的大力支持，研究范围从基础科学到翻译科学。这一新仪器将通过深入了解神经退行性疾病的分子基础、癌症诊断治疗以及药物发现的新工具，对人类健康产生广泛影响。

DESCRIPTION (provided by applicant): This application requests state-of-the-art components essential for 1) establishing biological solid-state NMR at UCSF; and 2) considerably enhancing current efforts focused on investigating metabolic pathways and biomarkers for human diseases. The needed instrument is a combined liquids and solid-state 600 MHz NMR spectrometer console and accessories that allow: 1) High-resolution triple-resonance magic-angle spinning experiments to perform conformational analyses of mammalian and fungal prion protein fibrils; 2) high-throughput, automated, multinuclear detection of cell extracts to establish correlations with NMR detectable biomarkers; and 3) development of new, ultra-sensitive NMR techniques to monitor ligand binding to macromolecules using Dynamic Nuclear Polarization. Each of these areas are heavily supported at the University of California by the NIH, with investigations spanning from basic to translational science. The new instrument will have a broad impact on human health by providing insight into molecular bases for neurodegenerative disease, diagnostic treatments for cancer, and new tools for drug discovery.