Becton Dickinson FACSAria IIu Cell Sorter Flow Cytometer

描述（由申请人提供）：当前提案的主要目标是获得购买Becton Dickinson FACSAria II细胞分选流式细胞仪（BD-FACS）系统的资金，包括专门设计用于分析人类和感染性材料的Becker BioPROtect III生物密封外壳。流式细胞术是一种独特而强大的技术，用于分析细胞的表型和功能，以及通过分选分离确定的细胞群。近年来，随着研究病毒与免疫系统细胞之间相互作用的新方法的发展，流式细胞术分析在病毒学研究中的应用变得越来越突出。基于荧光的报告系统已被开发用于监测病毒复制、再激活和重组，仪器仪表的先进技术进步为高速多色分析和细胞分选提供了新的平台。在癌症和干细胞研究中，利用流式细胞术进行细胞分选是确定和表征器官发育以及肿瘤起始、生长和转移的前体细胞的最先进方法。我们建议与乔治亚州医学中心（MCG）流式细胞仪核心设备（FACS Core）一起购买该仪器。该仪器最初将支持一个由至少18名已建立的研究人员组成的中心小组的几个项目，该小组由NIH拨款资助。这些项目的需求是多种多样的，包括FACS对病毒感染小鼠的免疫细胞群进行分选，纯化人类或小鼠干细胞（器官或肿瘤类型特异性），以及与病毒感染、癌症发展和神经退行性疾病相关的炎症细胞群。该仪器还可用于支持MCG许多其他研究人员的研究项目，特别是在需要对生物有害材料进行分类的程序中。因此，获得这种细胞分选系统将显著提高和加速nih资助项目的进展，特别是那些需要分析暴露于病原体（病毒）的人类和动物组织细胞群的分离和功能分析的项目。由于许多研究人员大量使用Core，它将大大提高MCG FACS设施在非传染性材料细胞分选方面的有限能力。我们还致力于通过与用户合作、促进合作和跨核心伙伴关系来促进创新，鼓励在传染病、癌症和神经退行性疾病以及干细胞研究方面进行尖端的基础和转化研究。

DESCRIPTION (provided by applicant): The major goal of the current proposal is to obtain funds for the purchase of a Becton Dickinson FACSAria II Cell Sorter Flow Cytometer (BD-FACS) system, including a Becker BioPROtect III Bio-containment enclosure that is specifically designed for analyses on human and infectious materials. Flow cytometry is a unique and powerful technology for analyzing the phenotype and function of cells, as well as for isolating defined cell populations by sorting. In recent years, the use of flow cytometric analyses in virology research has become ever more prominent as newer approaches for dissecting the interactions between viruses and cells of the immune system have been developed. Fluorescent-based reporter systems have been developed to monitor virus replication, reactivation, and recombination, and sophisticated technological advances in instrumentation have provided new platforms for high-speed polychromatic analyses and cell sorting. In cancer and stem cell research, cell sorting by flow cytometry is the state-of-the-art method to determine and characterize the precursor cells for organ development as well as for tumor initiation, growth, and metastasis. We propose to purchase this instrument in conjunction with the Medical of Georgia (MCG) Flow Cytometry Core Facility (FACS Core). The instrument will initially support several projects of a central group of at least 18 established investigators funded by NIH grants. The needs of these projects are varied and include FACS sorting of immune cell populations from virally infected mice, and the purification of human or mouse stem cells (organ or tumor-type-specific) as well as inflammatory cell populations associated with viral infections, cancer development, and neurodegenerative diseases. The instrument will be also available to support the research projects of many other investigators at MCG, especially in procedures that require sorting of biohazardous materials. Thus, the acquisition of this cell sorter system will significantly enhance and accelerate progress in NIH-funded projects, especially those in which separation and functional analysis of cell populations from human and animal tissues exposed to pathogens (viruses) need to be analyzed. It will significantly elevate the limiting capacity of the MCG FACS facility in cell sorting of non-infectious materials due to the Core's heavy use by many investigators. We also aim to promote innovation by working with users and fostering collaborations and cross-core partnerships that encourage cutting-edge basic and translational research in infectious diseases, cancer, and neurodegenerative diseases as well as stem-cell research.