Confocal Laser Scanning Microscope

描述（由申请人提供）：该提案申请资金用于购买共享使用的共聚焦激光扫描显微镜系统，LSM 710配置#4 3ch 405， Ar, 561, 633 ZEN。蔡司LSM 710共聚焦激光扫描显微镜系统具有三个光谱检测通道/ pmt，以及6条激光线的组合- 405、458,514、488,561和633 nm作为激发源，允许在不同光谱范围内同时观察荧光探针。高度精密的激光扫描仪器，用于成像固定的生物标本以及活细胞和组织，利用最先进的荧光探针进行多重标准共聚焦成像应用。该生物分析系统将服务于密苏里大学（UMKC）医学院越来越多的nih资助的研究人员。该系统将满足当前和未来对先进成像的需求，并使医学院的教学技术现代化。当前共聚焦显微镜的研究需要包括：1)测量活细胞中信号分子的亚细胞变化；2)以常规荧光显微镜无法达到的分辨率对新分离的细胞和组织进行光学切片；3)确定低丰度蛋白质随时间的分布，同时尽量减少对细胞生理的影响；4)阐明基因修饰细胞中蛋白表达和转运的机制；5)对新型药物进行体内外构效关系分析。目前的应用程序将主要服务于主要研究人员周围的研究小组，这些研究小组由来自不同NIH研究所的同行评审拨款外部资助。美国国立卫生研究院资助的医学院研究人员需要这种仪器，他们对影响美国人口的疾病进行研究，这些疾病的影响很大，而且越来越多，包括受医疗保健服务差异影响的少数民族。拟议的设备将加速研究进展，提高研究成果的质量和广度，确定病因、作用机制，并随后有助于发现潜在的治疗战略和改善保健。

DESCRIPTION (provided by applicant): The proposal requests funding for the acquisition of a shared-use confocal laser-scanning microscope system, a LSM 710 configuration #4 3ch 405, Ar, 561, 633 ZEN. The Zeiss LSM 710 Confocal Laser Scanning Microscope System with three spectral detection channels / PMTs, and a combination of six laser lines - 405, 458, 514, 488, 561 & 633 nm as excitation sources allowing the simultaneous observation of fluorescent probes in distinct spectral ranges had been specifically developed as a state-of-the-art, highly sophisticated laser scanning instrument to image fixed biological specimens as wells as living cells and tissues utilizing state-of-the-art fluorescent probes for multiple standard confocal imaging application. This bioanalytical system will serve an increasing community of NIH-funded researchers at the University of Missouri (UMKC) School of Medicine. The system will meet the current increased and future needs for advanced imaging, as well as modernize the School of Medicine's teaching technology. Current research needs for a confocal microscope include: 1) measuring subcellular changes in signaling molecules in live cells; 2) optical sectioning of freshly isolated cells and tissue at a resolution that cannot be achieved with conventional fluorescence microscopy; 3) determining the distribution of low-abundance proteins over time while minimally affecting cell physiology; 4) elucidating mechanisms protein expression and trafficking in genetically modified cells; 5) conducting analyses of structure-activity relationships of novel agents in vitro and in vivo. The current application will primarily serve research groups around principal investigators that are extramurally funded by peer-reviewed grants from different NIH institutes. NIH-funded researchers at the School of Medicine in need of this instrumentation conduct research on diseases that affect significant and increasing portions of the U.S. population including minorities affected by disparities in health care delivery. The proposed equipment will accelerate research progress and enhance the quality and breadth of research results determining causes, mechanisms of action and subsequently contributing to discovering potential treatment strategies and improving health care.