Femtosecond Infrared Laser Ablation Platform

描述(由申请人提供)：申请资金用于飞秒红外激光消融(FIRLA)平台，用于与位于波士顿大学生物金属和金属组学中心的NCRR资助的高分辨率扇区场电感耦合等离子体质谱仪(SF-ICPMS)耦合(联用)。Firla-SF-ICPMS联用系统将实现前所未有的超痕量元素和同位素图谱研究，由代表该地区和全球领先机构的40多个创新研究团队进行。该联用系统将是全国首个此类专用生物金属组学资源。飞秒激光消融技术提供了前所未有的空间分辨率，极大地提高了分析的准确度和精确度，并将附带的热损害降至最低，从而首次在微米分辨率下对生物样品进行了超痕量元素和同位素图谱研究。所要求的共享资源将促进波士顿大学医学院、波士顿大学公共卫生学院、哈佛医学院、哈佛公共卫生学院、马萨诸塞州总医院、布里格姆妇女医院、美国地质服务局、武装部队放射生物学研究所、劳伦斯·伯克利国家实验室、爱尔兰博物馆、女王大学和墨尔本大学的领先跨学科团队开展的广泛创新研究。在NCRR支持的生物金属和金属组学中心部署建议的联用仪器将加快在阿尔茨海默病、帕金森病、创伤性脑损伤、眼睛疾病、环境毒理学、癌症生物学、微量营养素生理学、败血症和炎症、生物金属组骨考古学、法医病理学、军事医学和纳米疗法方面的领先发现。申请的仪器将作为与波士顿大学其他地区资源结盟的共享资源进行管理，这些资源包括NCRR质谱学资源、NIA阿尔茨海默病中心、NCRR临床和翻译科学研究所、Framingham研究和波士顿大学光子学中心。CBM的工作人员是一支在生物医学金属分析、激光烧蚀、激光诱导击穿光谱、元素和同位素电感耦合等离子体质谱以及光学发射光谱方面拥有20多年经验的专家分析团队。首席调查员和咨询委员会将促进公平利用这一独特的分析资源，并使其具有成本效益。这一共享的仪器和联合的核心资源将促进建立国家冶金组学英才中心，并促进这一重要新领域的前沿科学发现。 与公共健康相关：波士顿大学NCRR资助的生物金属与金属组学中心申请了用于金属组织测绘的超快激光的资金。这一共享资源将支持阿尔茨海默氏症、帕金森氏病、创伤性脑损伤、肺癌、环境毒理学和纳米医学方面的开创性研究。

DESCRIPTION (provided by applicant): Funds are requested for a femtosecond infrared laser ablation (FIRLA) platform for coupling (hyphenation) to the NCRR-funded high-resolution sector field ICP mass spectrometer (SF-ICP-MS) located at the Center for Biometals and Metallomics at Boston University. The hyphenated FIRLA-SF-ICP-MS system will enable unprecedented ultra-trace elemental and isotopic mapping studies conducted by over forty innovative research teams representing leading institutions throughout the region and across the globe. The hyphenated system will be the first such dedicated biometallomic resource of its kind in the nation. Femtosecond laser ablation technology provides unprecedented spatial resolution with greatly improved analytical accuracy and precision with minimal collateral thermal damage, thus affording for the first time ultra-trace elemental and isotopic mapping studies in biological specimens at micron resolution. The requested shared resource will facilitate a wide range of innovative research conducted by leading interdisciplinary teams at Boston University School of Medicine, Boston University School of Public Health, Harvard Medical School, Harvard School of Public Health, Massachusetts General Hospital, Brigham & Women's Hospital, United States Geological Service, Armed Forces Radiobiology Research Institute, Lawrence Berkeley National Laboratory, Museum of Ireland, Queen's University, and the University of Melbourne. Deployment of the proposed hyphenated instrument at the NCRR-supported Center for Biometals and Metallomics will accelerate lead-edge discovery in Alzheimer's disease, Parkinson's disease, traumatic brain injury, ocular disorders, environmental toxicology, cancer biology, micronutrient physiology, sepsis and inflammation, biometallomic osteoarcheology, forensic pathology, military medicine, and nanotherapeutics. The requested instrument will be managed as a shared resource allied to other regional resources at Boston University, including the NCRR Mass Spectrometry Resource, NIA Alzheimer's Disease Center, NCRR Clinical and Translational Science Institute, the Framingham Study, and the Boston University Photonics Center. The CBM is staffed by an expert analytical team with over two decades of experience in biomedical metallomic analyses, laser ablation, laser-induced breakdown spectroscopy, elemental and isotopic ICP-MS, and optical emission spectroscopy. The Principal Investigator and Advisory Committee will promote equitable access and cost-effective utilization of this unique analytical resource. This shared instrument and allied core resource will facilitate establishment of a National Center of Excellence in metallomics and catalyze scientific discovery at the forefront of this important new field. PUBLIC HEALTH RELEVANCE: Funds are requested for an ultra-fast laser for metallomic tissue mapping at the NCRR-funded Center for Biometals & Metallomics, Boston University. This shared resource will support pioneering research in Alzheimer's disease, Parkinson's disease, traumatic brain injury, lung cancer, environmental toxicology, and nanomedicine.