Fluorescence Microscope Application

描述（由申请人提供）：奥克兰大学的五名美国国立卫生研究院资助的研究人员（拥有五项目前资助的NIH资助和一项未决资助）申请资金购买蔡司电动Axio成像仪。Z1荧光显微镜。所要求的仪器将取代研究人员目前使用的已有12年历史、几乎过时的尼康荧光显微镜。新仪器将大大提高参与研究人员完成NIH资助目标的能力，并跟踪任何潜在的令人兴奋的新线索。使用新仪器进行的荧光分析类型包括晶状体、视网膜和软骨中的各种类型的免疫定位分析，晶状体上皮中活细胞和死细胞的检测，以及组织中DNA单链断裂的测定，晶状体中谷胱甘肽蛋白的鉴定，以及视网膜中生物分子荧光互补的测定。拟议工作的长期目标是确定原因，并寻求治疗某些常见的衰老疾病，包括青光眼，白内障，年龄相关性视网膜变性和骨关节炎。这些疾病中的每一种都是美国老年人口的主要健康问题。

DESCRIPTION (provided by applicant): A group of five NIH-funded investigators at Oakland University (possessing five currently-funded NIH grants and one pending) request funds to purchase a Zeiss Motorized Axio Imager. Z1 Fluorescence Microscope. The requested instrument will replace a 12-year old, nearly obsolete Nikon fluorescence microscope that is currently being used by the researchers. The new instrument will greatly enhance the ability of the participating investigators to complete the aims of their NIH grants, and to follow any potentially exciting new leads. Types of fluorescence assays to be conducted with the new instrument include various types of immunolocalization assays in lens, retina and cartilage, detection of live and dead cells in lens epithelium, as well as determination of DNA single-strand breaks in that tissue, identification of glutathiolated proteins in lens, and assay of biomolecular fluorescence complementation in retina. The long-term goals of the proposed work are to determine the causes, and seek cures for certain common diseases of aging including glaucoma, cataract, age-related retinal degeneration and osteoarthritis. Each of these diseases represents a major health concern for the elderly population of the United States.