LTQ Orbitrap XL ETD Mass Spectrometer

描述（由申请人提供）：斯坦福大学是一些世界上最先进的生物和医学研究的所在地。蛋白质组学作为一种研究应用，为基本的生物学问题提供有见地的答案，现在是一个先决条件。蛋白质组学的基石之一，如果不是基础的话，是质谱。在过去的五年中，与计算资源并行的质谱仪器的技术发展已经引起了酵母蛋白质组的完全鉴定和定量，人类组蛋白及其同种型的完全翻译后映射，以及映射信号事件（磷酸化蛋白质组）作为对全球蛋白质组规模上的刺激的响应的能力。这些深刻的发现与现代仪器的能力密不可分。该提案寻求资金，以协助购买一台新的LTQ Orbitrap XL ETD质谱仪。轨道阱将在斯坦福大学的质谱核心设施斯坦福大学质谱中心（SUMS）进行专业操作和维护。SUMS目前的1年用户群包括来自26个部门的80个研究小组的245名学生，教师和工作人员，这些部门分布在医学院（60%），人文科学院（30%）和工程学院（10%）。轨道阱质谱仪的能力得到了证明，操作仪器并将所得数据转化为具有生物学意义的结果的技术专长已经存在于SUMS中。轨道阱的加入将使目前需求量很大的研究项目变得可行。在许多其他应用中，该仪器的高质量精度和灵敏度将使对癌细胞、干细胞和胎鼠血清的定量蛋白质组学研究成为可能。ETD的能力将被用于植物细胞信号传导和肿瘤发生中涉及的蛋白质的磷酸化蛋白质组学表征。此外，该仪器将能够对病毒进展至关重要的蛋白质进行自上而下和自下而上的表征。如提案所述，七个主要用户的研究项目与人类健康和疾病高度相关，对病毒和细菌感染、干细胞和细胞命运以及几种癌症具有特别影响。这些项目代表了校园内许多研究人员所面临的挑战。 公共卫生相关性：蛋白质组学是在最广泛的范围内研究蛋白质，确定有关蛋白质的最基本信息：什么，谁，在哪里和为什么对理解疾病和疾病状态有影响。质谱法是一种已经解决并将继续明确回答这些问题的工具。将LTQ Orbitrap XL ETD质谱仪的成熟功能应用于本提案中描述的研究，有可能影响人类健康和疾病，推动许多领域的研究向前发展，包括病毒和细菌感染，干细胞和细胞命运，以及几种类型的癌症。

DESCRIPTION (provided by applicant): Stanford University is home to some of the world's most progressive biological and medical research. Proteomics as a research application, providing insightful answers to fundamental biological questions, is now a prerequisite. One of the cornerstones of proteomics, if not the foundation, is mass spectrometry. The technological development of mass spectrometric instrumentation in parallel with computational resources over the past five years has given rise to the complete identification and quantification of the yeast proteome, complete post-translational mapping of human histones and isoforms thereof, as well as the ability to map signaling events (phosphoproteome) as a response to stimulation on a global proteomic scale. These profound findings are inextricably linked to the capabilities of modern instrumentation. This proposal seeks funds to assist in the purchase of a new LTQ Orbitrap XL ETD mass spectrometer. The Orbitrap will be professionally operated and maintained in Stanford's mass spectrometry core facility, Stanford University Mass Spectrometry (SUMS). SUMS' current 1-year user base includes 245 students, faculty, and staff in 80 research groups from 26 departments distributed across the schools of Medicine (60%), Humanities and Sciences (30%), and Engineering (10%). The capabilities of the Orbitrap mass spectrometer are proven, and the technical expertise to operate the instrumentation and translate the resulting data into biologically meaningful results is already present in SUMS. The addition of the Orbitrap would make research projects that are now in high demand viable. Amongst many other applications, the high mass accuracy and sensitivity of the instrument would enable quantitative proteomic studies on cancer cells, stem cells, and fetal mouse serum. The capabilities of ETD would be utilized in phosphoproteomic characterization of proteins involved in plant cell signaling and tumorgenesis. In addition, the instrumentation would enable top-down and middle-down characterization of proteins critical to viral progression. The research projects of seven major users, as described in the proposal, are highly relevant to human health and disease, with particular impacts on viral and bacterial infections, stem cells and cell fate, and several types of cancers. These projects are representative of the types of challenges faced by many researchers on campus. PUBLIC HEALTH RELEVANCE: Proteomics is the study of proteins on the broadest scale, determining the most basic information about proteins: the what, who, where and why have implications in understanding disease and disease states. Mass spectrometry is one tool which has addressed and will continue to answer these questions definitively. Applying the proven capabilities of the LTQ Orbitrap XL ETD mass spectrometer to the research described in this proposal has potential to impact human health and disease, moving research forward in numerous areas, including viral and bacterial infections, stem cells and cell fate, as well as several types of cancers.