Illumnia Genome Analyzer II

描述（由申请人提供）：这是一项在罗切斯特大学建立高通量DNA测序技术的提案。该大学拥有丰富的NIH资助的研究组合，这些研究将受益于高通量DNA测序。从致龋细菌的基因组分析到遗传性心脏病患者的长QT等位基因绘图等各种项目，将解决对人类健康有直接影响的问题。进一步的项目包括癌基因合作，代谢调控，信号转导，基因调控和表观遗传学的研究。这些项目中的许多项目的PI都是其领域内国际公认的领导者，并在最具影响力的期刊上发表他们的工作。此外，由罗切斯特大学科学家协调的多校区项目Nasonia（一种寄生蜂）基因组项目将从这项技术中受益匪浅。此外，具有生物信息学和生物统计学经验的数学和统计团队将开发和完善创新工具，用于将高通量测序技术应用于生物医学研究。所需仪器为Illumina Genome Analyzer II。它将被安置在已建立的功能基因组学核心设施（FGC）中。FGC的一名全职专职工作人员将运行仪器。FGC的工作人员还将在数据处理和分析方面为用户提供帮助。新落成的罗切斯特大学计算研究中心（CRC）将提供更先进的计算支持，该中心拥有几个高性能计算和大型数据存储资源。CRC的工作人员包括具有基因组学专业知识的程序员和信息学家，他们将协助用户分析大规模DNA序列数据。将为该工具的运行提供机构支持和监督。将基因组核心服务扩展到包括高通量测序是罗切斯特大学战略计划的一个组成部分，该计划预计在未来四年内基因组学研究将大幅扩展。该战略计划包括在基因组学，系统生物学和生物信息学领域招聘10名新教师。此外，还将扩大生物信息学和基因组学的本科生和研究生教学。

DESCRIPTION (provided by applicant): This is a proposal to establish high throughput DNA sequencing technology at the University of Rochester. The University has a rich portfolio of NIH-funded research that would benefit from access to high throughput DNA sequencing. Diverse projects, ranging from genome analysis in caries-causing bacteria to mapping of long QT alleles in patients with hereditary heart conditions, will address questions with direct impact on human health. Further projects include research on oncogene cooperation, metabolic regulation, signal transduction, gene regulation and epigenetics. The PIs of many of these projects are internationally recognized leaders in their fields and publish their work in the journals of the highest impact. In addition, the Nasonia (a parasitic wasp) genome project, a multi-campus effort that is coordinated by scientists at the University of Rochester would benefit substantially from this technology. Moreover, mathematical and statistical teams with experience in bioinformatics and biostatics will develop and refine innovative tools for the application of high throughput sequencing technology to biomedical research. The requested instrument is an Illumina Genome Analyzer II. It will be housed in the established functional genomics core facility (FGC). A full-time dedicated staff member of the FGC will run the instrumentation. Staff of the FGC will also provide user assistance with data processing and analysis. More advanced computational support will be available through the newly inaugurated University of Rochester Center for Research Computing (CRC), which houses several high performance computing and large data storage resources. The staff of the CRC includes programmers and informaticians with expertise in genomics, who will be available to assist users with the analysis of large scale DNA sequence data. Institutional support and oversight will be provided for the running of the instrument. The extension of genomic core services to include high throughput sequencing is an integral part of the strategic plan of the University of Rochester, which projects a significant expansion of genomics research over the coming four years. This strategic plan includes the recruitment of ten new faculty members in the areas of genomics, systems biology, and bioinformatics. In addition, undergraduate and graduate teaching in bioinformatics and genomics will be expanded.