LTQ-Orbitrap-XL-ETD Mass Spectrometer for LLU Mass Spectrometry Facility

描述(申请人提供)：摘要本申请申请资助一台最先进的共享式质谱仪，该质谱仪将为洛马林达大学的质谱学设施提供合作研究。LTQ-Orbitrap-XL-ETD质谱仪由Thermo Fisher Science制造。LTQ-Orbitrap-XL-ETD LC/MS/MS系统提供了解的灵敏度、分辨率、质量测量精度和宽动态范围特性。它结合了三种不同和互补的碎片技术CID、HCD和ETD，以及Orbitrap技术的优势，为复杂的PTM分析、多肽的智能测序、自上而下和中间向下的分析以及通过无标记或稳定的同位素标记(如iTRAQ和TMT)进行蛋白质定量提供了最全面的解决方案。由于购买这台质谱仪的成本为729,000美元，我们向NIH共享仪器项目申请499,999.23美元，洛马林达大学医学院将承诺229,000.77美元。这台质谱仪将支持洛马琳达大学九个系或中心的11名NIH主要用户和来自附近研究所的两名主要用户，他们是加州大学洛杉矶分校的Michael Grunstein博士和UCR的朱建康博士，他们将与我们的设施合作，继续他们的表观遗传学研究。30多名研究人员(主要用户和次要用户)将受益于这一工具，其中大多数人持有国家卫生研究院的赠款。随着这台仪器的收购，我们NIH的主要用户的研究将在疾病和癌症、神经元损伤和新生儿疼痛的生物标记物识别、了解DNA损伤和修复所涉及的机制、传染病的病理学以及表观遗传学和癌症研究中的组蛋白修饰等领域取得显著进展。 公共卫生相关性：我们要求LTQ-Orbitrap-XL-ETD质谱仪，以满足我们NIH赞助的用户在表观遗传学、传染病、神经元损伤、疼痛、缺氧应激和人类癌症等方面的研究进展。

DESCRIPTION (provided by applicant): Summary This application requests funding for a state-of-the-art shared mass spectrometer that will provide collaborative research for the Loma Linda University mass spectrometry facility. The LTQ-Orbitrap-XL-ETD mass spectrometer is manufactured by Thermo Fisher Scientific. The LTQ-Orbitrap-XL-ETD LC/MS/MS system provides understanding sensitivity, resolving power, accuracy of mass measurement, and wide dynamic range characteristics. It combines three different and complementary fragmentation techniques CID, HCD, and ETD with the proven benefits of Orbitrap technology for most comprehensive solution for complex PTM analysis, intelligent sequencing of peptides, top-down and middle-down analysis, and protein quantification via label-free or stable isotope labeling such as iTRAQ and TMT. As the cost of purchasing this mass spectrometer is $729,000, we request $499,999.23 from NIH shared instrumentation program and the School of Medicine of Loma Linda University will commit $229,000.77. This mass spectrometer will support 11 major NIH users in nine departments or centers at Loma Linda University and 2 major users from nearby institutes, Dr. Michael Grunstein at UCLA and Dr. Jian-Kang Zhu at UCR, who will collaborate with our facility to pursue their research in epigenetics. More than 30 investigators (major users and minor users), majority of whom holds NIH grants, will be benefited from this instrument. With acquisition of this instrument our major NIH users' research will be significantly advanced in the areas including identification of biomarkers in disease and cancer, neuron-injury and neonatal pain, understanding the mechanisms involved in DNA damage and repair, pathology of infectious disease, and histone modifications in epigenetic and cancer studies. PUBLIC HEALTH RELEVANCE: We request a LTQ-Orbitrap-XL-ETD mass spectrometer to meet the needs of our NIH sponsored users to advance their research in epigenetics, infectious disease, neuron injury, pain, hypoxia stress and human cancers, etc.