Typhoon Trio Phosphor Imaging System

描述（由申请人提供）：本建议书旨在获得购买台风三人成像仪的资金。这种成像仪允许捕获、图像构建、定量分析和从多种形式的分子、生物和生化实验中生成出版物图像，包括但不限于南方和北方印迹、DNA结合测定、DNA足迹、蛋白质凝胶和免疫印迹。台风三重奏能够在放射自显影、化学发光和荧光介质上执行这些操作，允许对生成的数据进行快速有效的定量。台风三人组允许在一次实验中分析多个参数。技术范围从南部和北部印迹，EMSA， DNA足迹，多色免疫印迹，二维分离，薄层色谱将成像和定量使用台风三重奏。在这个应用程序中，已经组建了一个由五个部门的18个生产实验室组成的用户组。该组的研究活动范围广泛，包括传染病免疫反应的调节、自身免疫、细菌和病毒发病机制、发育和生育、基因组完整性和基因调节、人类遗传疾病和代谢紊乱、智力迟钝、肌肉再生、疫苗开发和视力障碍等方面的研究。总的来说，这些研究领域的目的是了解和寻找癌症、传染病、治疗儿童和成人遗传疾病以及肌肉萎缩的治疗方法。仪器的高通出率、较短的设置和训练时间，以及“台风三人组”所产生的高质量数据，将提高这支多元化调查队伍的科学生产力。

DESCRIPTION (provided by applicant): This proposal seeks to obtain funds to purchase a Typhoon Trio Imager. Such imagers allow the capture, image construction, quantitative analyses, and production of publication images from numerous forms of molecular, biological, and biochemical experiments, including but not limited to Southern and northern blots, DNA binding assays, DNA footprinting, protein gels, and immunoblots. The Typhoon Trio is able to perform these operations on autoradiographic, chemiluminescent, and fluorescent medias, allowing rapid and efficient quantitation of the data generated. The Typhoon Trio allows multiple parameters to be analyzed in a single experiment. Technologies ranging from Southern and northern blotting, EMSA, DNA footprinting, multi-color immunoblotting, two-dimensional separations, to thin layer chromatography will be imaged and quantitated using the Typhoon Trio. In this application, a user group of 18 productive laboratories in five departments has been assembled. The research activities of this group is broad and includes investigation into the regulation of the immune response in infectious disease, autoimmunity, mechanisms of bacterial and viral pathogenesis, development and fertility, genome integrity and gene regulation, human inherited diseases and metabolic disorders, mental retardation, muscle regeneration, vaccine development, and vision disorders. Broadly, these areas of investigation are aimed at understanding and finding cures for cancer, infectious disease, treating childhood and adult inherited diseases, and muscle wasting. The high through put of the instrumentation, the short set up and training time, and the quality of the data produced by the Typhoon Trio will increase the scientific productivity of this diverse group of investigative teams.