GE TYPHOON 9410 VARIABLE MODE IMAGER

描述(由申请人提供)：成像和量化标记分子是许多现代生物医学研究技术的关键组成部分。在过去的11年里，我们对南方和北方杂交的结果以及分子动力学磷光成像仪SI上的核糖核酸酶保护进行了成像。结果必须使用运行过时的System 8软件的旧Macintosh计算机上的软件进行分析。如果磷成像仪或计算机出现故障，它们将无法维修。在使用了11年后，磷成像屏幕的使用寿命已经超过了它们的使用寿命。麦克劳克林研究所是一家独立的非营利性研究机构，位于蒙大拿州的大瀑布，距离其他具有荧光粉成像能力的设施有三个小时的车程。虽然Southern和Northern杂交实验继续依赖于放射性同位素标记，但荧光标记技术继续进步，在核酸和蛋白质成像方面有新的应用。我们需要用一台能够成像和量化放射性和荧光标记分子的荧光粉成像仪来取代我们的成像仪。我们请求使用通用电气9410台风可变模式成像仪。除了作为传统的荧光粉成像器，台风9410还可以探测红色、绿色和蓝色的荧光，允许同时检测多个核酸和蛋白质。这种荧光能力将允许我们执行多重PCR反应进行基因分型，以及检测从丙烯酰胺凝胶(蛋白质印迹)或从组织切片(组织印迹)转移到膜上的蛋白质。台风9410有10微米的分辨率，这是一个特别有用的功能。该设备提供的最新荧光粉成像能力将使我们能够更高效、更可靠地使用放射性标记核酸进行实验，但也将扩大我们使用标记蛋白质执行最先进技术的能力。 公共卫生相关性：为了对对我们的研究至关重要的放射性标记分子进行成像和量化，我们依赖于分子动力学磷光成像仪SI，其能力在过去11年中显著恶化。尽管放射性同位素标记仍然是一种有用的研究技术，但荧光标记技术仍在不断进步。我们需要更换我们的 荧光粉成像仪，可对放射性和荧光进行成像和定量 标记分子来推进我们的研究，这与神经退行性疾病有关， 帕金森氏症和髓鞘疾病。

DESCRIPTION (provided by applicant): Imaging and quantifying labeled molecules is a critical component of many modern biomedical research techniques. For the last 11 years we have imaged the results of Southern and northern hybridizations, and RNAse protections on a Molecular Dynamics Phosophorimager SI. The results must be analyzed using software on an old Macintosh computer that runs obsolete System 8 software. Should the phosphorimager or computer fail, they cannot be serviced. After 11 years of use, the phosphorimaging screens have deteriorated past their useful lifetime. The McLaughlin Research Institute is an independent non-profit research institute in Great Falls, Montana, three hours' drive away from other facilities with phosphor imaging capability. Although Southern and northern hybridization experiments continue to rely on radio isotopic labeling, fluorescent labeling techniques continue to advance, with new applications in imaging nucleic acids and proteins. We need to replace our phosphor imager with one that is capable of imaging and quantifying both radioactive and fluorescently labeled molecules. We are requesting the General Electric Typhoon 9410 Variable Mode Imager. In addition to functioning as a traditional phosphor imager, the Typhoon 9410 can detect red, green and blue fluorescence, permitting multiple nucleic acids and proteins to be detected simultaneously. This fluorescent capability will permit us perform multiplex PCR reactions for genotyping, as well as detection of proteins transferred to membranes from acrylamide gels (western blots) or from tissue sections (histoblots). The Typhoon 9410 has 10 micron resolution, a particularly useful feature for histoblots. The updated phosphor imaging capabilities that are provided by this equipment will permit us to more efficiently and reliably perform experiments using radio labeled nucleic acids, but will expand our capability to perform state-of-the-art techniques using labeled proteins as well. Public Health Relevance: To image and quantify radiolabelled molecules that are essential for our research, we have relied on a Molecular Dynamics Phosophorimager SI, whose capabilities have deteriorated considerably over the last 11 years. Although radioisotope labeling remains a useful research technique, fluorescent labeling techniques continue to advance. We need to replace our Phosphor imager with one that is capable of imaging and quantifying both radioactive and fluorescently Labeled molecules to advance our research, which is relevant to neurodegenerative diseases, Parkinson's disease and myelin diseases.