Shared LSRII Cytometer Equipment Application - UC Davis Stem Cell Program

多色流式细胞术已成为许多研究领域的强大工具，自2000年以来，加州大学戴维斯分校一直处于该技术的最前沿。随着第一个定制设计的12色，14参数MoFlo细胞分选机的开发，UCD研究人员一直在开发多色面板用于研究。这项研究已经产生了250多篇出版物和200多项NIH赠款，其中仅今年就有57项NIH赠款。UCD Opticore于1995年在戴维斯校区成立，于2002年扩展到萨克拉门托的NCI指定癌症中心，并于2006年再次扩展到与新的干细胞计划合作的第二个萨克拉门托位置。萨克拉门托的设施可以访问各种各样的计划，包括UCD医疗中心，MIND研究所，干细胞计划和癌症中心。目前，26个国家卫生研究院在加州大学萨克拉门托分校的赠款依赖于获得多参数流式细胞仪。然而，这些项目仅限于使用为干细胞生物学项目购买的细胞分选仪。该分拣机需要专门的操作员，已经超过50%的用户容量，并且在设施开放后仅一年就继续增长。获得自主操作的多参数分析流式细胞仪对于NIH资助的26个研究项目的成功至关重要，其中许多项目不需要常规的细胞分选。LSR-II细胞仪是唯一的台式分析仪器，具有五种激光能力，能够采集多达18个参数，包括16种不同的荧光染料，并具有未来扩展的额外能力。这种最先进的仪器将使我们能够轻松转移已经开发的分析面板，并添加本申请中所述的特定研究项目所需的其他应用和荧光染料。将仪器放置在光学生物学核心设施将确保仪器得到仔细维护，研究人员将最大限度地获得流式细胞仪仪器和专业知识。

DESCRIPTION (provided by applicant): Multicolor flow cytometry has become a powerful tool in many areas of research, and UC Davis has been at the forefront of this technology since 2000. With the development of the first custom designed 12-color, 14 parameter MoFlo Cell Sorter, UCD investigators have been developing multi-color panels for research. This research has generated over 250 publications and more than 200 NIH grants, including 57 NIH grants this year alone. The UCD Opticore was formed in 1995 on the Davis Campus, expanded to the NCI designated Cancer Center in Sacramento in 2002, and expanded again in 2006 to a second Sacramento location in collaboration with the new Stem Cell Program. The Sacramento facilities are accessible to a wide range of programs, including the UCD Medical Center, the MIND Institute, the Stem Cell Program, and the Cancer Center. Currently, 26 NIH grants based at UCD-Sacramento are dependent on access to a multi- parameter flow cytometer. However, these projects are limited to the use of a cell sorter, which was purchased for the Stem Cell Biology program. This sorter requires a dedicated operator, is already at greater than 50% user capacity and continuing to grow only a year after the facility opening. The acquisition of a self-operated, multi-parameter analytical flow cytometer will be critical to the success of the 26 NIH-funded research programs, many of which do not require cell sorting on a routine basis. The LSR-II cytometer is the only benchtop analysis instrument with five laser capability and the capacity to acquire up to 18 parameters, including 16 different fluorochromes, with additional capacity for future expansion. This state-of-the-art instrument will allow us to readily transfer the analytical panels already developed, plus add the additional applications and fluorochromes required for specific research programs as described in this application. Placing the instrument in the Optical Biology Core facility will ensure that the instrument is carefully maintained, and investigators will have maximum access to flow cytometry instrumentation and expertise.