COPAS BIOSORT Flow Cytometer

描述(申请人提供)：在过去的30年里，荧光激活细胞分选技术在许多领域取得了无数突破。在一次激动人心的技术飞跃中，这一概念最近被扩展到包括使用复杂对象参数分析仪和分类器(COPAS)对大型细胞、细胞颗粒甚至整个生物体进行分选。这项新兴技术已经在模式生物秀丽线虫中产生了重大影响，最近，Joslin糖尿病中心的研究人员与Copas制造商(Union Biometrica)合作，优化了对大型哺乳动物细胞或聚集体(脂肪细胞、类胚体、胰腺细胞聚集体等)的使用。这项工作是在暂时放置在Joslin的流式细胞仪核心设施中的演示仪器上进行的。由于这一技术优化阶段现已完成，该公司将在2009年夏季结束前拆除这些仪器。我们建议在这里购买一台COPAS BIOSORT仪器，该仪器将永久放置在Joslin Flow细胞分析仪核心中。该仪器将用于促进和扩大在临时演示机器上启动的研究工作。将购买的COPA将用于对秀丽线虫、上述哺乳动物细胞类型、造血干细胞/利基细胞聚集体和视网膜微血管进行分类。这些计划中的项目绝大多数是由美国国立卫生研究院资助的。预计该仪器将被大量使用，因为它将是波士顿朗伍德医学区唯一的此类仪器，该地区包括哈佛医学院及其许多主要附属医院研究机构。

DESCRIPTION (provided by applicant): During the last 30 years, the technology of fluorescence-activated cell sorting has enabled countless breakthroughs in many fields. In an exciting technological leap, this concept has recently been extended to include sorting of large cells, cellular particles, and even whole organisms by the Complex Object Parametric Analyzer and Sorter (COPAS) instrument. This emerging technology has already had significant impact in the model organism C. elegans, and recently investigators at the Joslin Diabetes Center have worked with the COPAS manufacturer (Union Biometrica) to optimize its use for large mammalian cells or aggregates (adipocytes, embryoid bodies, pancreatic cell aggregates, and others). This work has been performed on demonstration instruments that were placed temporarily in the Flow Cytometry Core facility at Joslin. As this technology optimization phase had now been completed, those instruments will be removed by the company before the end of Summer, 2009. We propose here to purchase a COPAS BIOSORT instrument that will be placed in the Joslin Flow Cytometry Core permanently. That instrument will be used to facilitate and expand research efforts that were initiated on the temporary demonstration machines. The COPAS to be purchased will be used to sort C. elegans, the mammalian cell types listed above, hematopoietic stem/niche cell aggregates, and retinal microvessels. The vast majority of these planned projects are NIH-funded. It is expected that this instrument will be used heavily, as it will be the only one of its kind in the Longwood Medical Area of Boston, which includes Harvard Medical School and many of its major affiliated hospital research facilities.