Biolmaging Core

该核心将为项目1、2和3提供最先进的生物成像支持。根德尔曼， 和Y.波斯斯基这项工作将包括定量磁共振成像（MRI），磁共振光谱成像（MRI）， 磁共振成像（MRSI）和单光子发射计算机断层扫描（SPECT）。两个7特斯拉的小动物 目前在内布拉斯加大学医学中心运行的MRI/S系统将提供定量的 神经影像学和超顺磁性氧化铁（SPIO）标记细胞追踪用于啮齿类神经艾滋病动物 HIV-1相关痴呆（HAD）模型。一个GammaMedica Ideas动物SPECT将用于 确定γ发射体标记的细胞或分子探针的生物分布，并提供了极好的 补充MRI细胞跟踪方法。图像处理实验室开发了定制的 MRI/SPECT动物固定器结合可见基准标记，以允许这两种标记的配准 方式。这允许SPECT图像的解剖细节和通过MRI的细胞定量的验证。 生物成像的核心方法还包括定量绘制血脑屏障通透性（项目 3、Y. Persidsky）、定量动脉自旋标记灌注成像和定量质子MRSI（1H MRSI）（项目2和3，H. Gendelman和Y. Persidsky）。此外，先进的成像和光谱 在MRI/S核心设施内已经开发了分析方法， 亚成像和共配准/扭曲成像结果与组织学（所有项目）。该核心还将 支持与HAD中小胶质细胞激活相关的发育治疗。这些长期目标 工作是评估生理MRI和生化1H MRSI神经病理学和细胞的预测因子， 迁移动力学（MRI和SPECT）在神经AIDS小鼠模型中的应用。这将提供敏感和 对疾病的发作和/或进展进行特定筛选，以及提供对 各种治疗干预。从该岩心获得的结果将直接适用于 监测人类神经退行性疾病的进程。核心的重大修改是 在焦点和设计上都做得很好，这样它就可以明确地提供新的数据，而这些数据不容易被 仅通过组织病理学检查获得。

This core will provide state-of-the-art bioimaging support for projects 1, 2, and 3, J. Zheng, H. Gendelman, and Y. Persidsky. The work will include quantitative magnetic resonance imaging (MRI), MR spectroscopic imaging (MRSI), and single photon emission computed tomography (SPECT). Two 7-Tesla small animal MRI/S systems, now operative at the University of Nebraska Medical Center, will provide quantitative neuroimaging and superparamagnetic iron oxide (SPIO) labeled cell tracking for rodent neuroAIDS animal models of HIV-1 associated dementia (HAD). One GammaMedica Ideas animal SPECT will be used in determining biodistribution of gamma emitter labeled cells or molecular probes and provides an excellent complement to MRI cell tracking methods. The image-processing laboratory has developed custom MRI/SPECT animal holders incorporating visible fiducial markers to allow coregistration of these two modalities. This allows anatomical details for SPECT images and validation of cell quantification by MRI. The bioimaging core methods also include quantitative mapping of blood-brain barrier permeability (project 3, Y. Persidsky), quantitative arterial spin labeled perfusion mapping, and quantitative proton MRSI (1H MRSI) (projects 2 and 3, H. Gendelman and Y. Persidsky). In addition, advanced imaging and spectroscopic analysis methods have been developed within the MRI/S core facility to allow automated mouse brain subimaging and coregistration/warping of imaging results with histology (all projects). This core will also support developmental therapeutics relevant to microglial activation in HAD. The long-term aims of these works are to assess the physiological MRI and biochemical 1H MRSI predictors of neuropathology and cell migration dynamics (MRI and SPECT) in mouse models of neuroAIDS. This will provide sensitive and specific screens of the onset and/or progression of disease as well as providing response kinetics to a variety of therapeutic interventions. The results obtained from this core will have direct applicability for monitoring the course of human neurodegenerative disorders. Significant modifications of the core were made in both focus and design so that it can unambiguously provide novel data that cannot readily be btained by histopathological examinations alone.