Molecular Core

这个项目的目标是研究传统和非传统线粒体的作用， 蛋白质在帕金森病（PD）的发病机制。分子核心的主要作用是（i） 提供个体项目所需的基因或基因产物，以及（2）改变表达 通过敲低（shRNA）或过表达其基因来研究蛋白质， PD的发病机制在个别项目中提出。 分子核心提供的服务包括：cDNA克隆，设计和生产构建体， 基因过表达或基因沉默，帮助产生瞬时和稳定转染， 用于体内基因转移的病毒载体的生产，以及含有蛋白质的融合蛋白的生产 转导结构域（达特）用于体外和体内有效的蛋白转导。这些服务将 这对成功完成每个项目至关重要。

The goal of this program is to investigate the roles of conventional and 'unconventional' mitochondrial proteins in the pathogenesis of Parkinson's disease (PD). The major roles of the Molecular Core are to (i) provide the genes or gene products that are required in the individual projects, and (2) alter the expression of proteins by either knockdown (shRNA) or overexpression of their genes to facilitate the investigation of the pathogenesis of PD proposed in the individual projects. Services provided by the Molecular Core include: cDNA cloning, design and production of constructs for gene overexpression or gene silencing, assistance with generation of transient and stable transfections, production of viral vectors for in vivo gene transfer, and generation of fusion proteins containing protein transduction domain (TAT) for efficient protein transduction in in vitro and in vivo. These services will be critical for successful completion of each of the individual projects.