EVAL OF NORMAL & ECTOPIC IMPLANTATION PRIMATES W CONTRAST ENHANCED ULTRASOUND

这个子项目是许多利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 我们的目标是进行恒河猴宫内早孕（IUP）的研究。在怀孕期间，子宫内膜的血管发生广泛的改变，为发育中的孕体提供血流。超声造影（CEU）是一种能够对器官内微血管进行高分辨率成像的新技术。该方法依赖于检测静脉注射的充气微泡，同时通过超声成像。 我们的假设是，CEU成像将允许早期可视化胚胎植入和第一次详细评估胎儿-母体界面的子宫内膜血管生长。我们的具体目标是开发微泡CEU，用于恒河猴早期妊娠的可视化和胚胎植入过程中宫内血管参数的评估。这一目标正在进行中，我们已经成像的子宫内膜在怀孕早期的5恒河猴。 我们的假设是，微泡CEU可能是一个有用的工具，在研究和早期诊断妊娠疾病，包括异位妊娠（EP）。 EP是一种严重的疾病，其中发育中的孕体附着并植入子宫外，最常见的是输卵管内。 早期诊断是成功治疗EP的关键，我们计划探索一种新的实验诱导的恒河猴EP模型。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Our goal is to perform studies on early intrauterine pregnancy (IUP) in rhesus macaques. During pregnancy the vessels of the endometrium are extensively modified to provide blood flow to the growing conceptus. Contrast-enhanced ultrasound (CEU) is a new technique capable of high-resolution imaging of the microvasculature within organs. The method relies on the detection of gas-filled microbubbles that are injected intravenously while imaging by ultrasound. Our hypothesis is that CEU imaging will permit earlier visualization of embryo implantation and for the first time a detailed evaluation of the growing endometrial vessels at the fetal-maternal interface. Our Specific Aim is to develop microbubble CEU for visualization of early pregnancy and assessment of intrauterine vascular parameters during embryo implantation in rhesus macaques. This aim is underway and we have imaged the endometrium during early pregnancy in 5 rhesus macaques. An extension of our hypothesis is that microbubble CEU may be a useful tool in the study and early diagnosis of pregnancy disorders including ectopic pregnancy (EP). EP is a serious condition where a developing conceptus attaches and implants outside the uterus, most frequently within the fallopian tube. Early diagnosis is essential for successful treatment of EP, and we plan to explore a new model for experimentally-induced EP in rhesus macaques.