DEVELOPMENT OF CORE SERVICE FOR CELLULAR IMMUNE ASSAYS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 细胞免疫学核心应要求为内部和外部研究人员的特定研究项目提供免疫学服务。目前的服务包括在最佳检测分析的标准化程序下的样品制备、ELISPOT检测的规划和执行、数据处理和演示、细胞内细胞因子染色，以及在这些检测中用作抗原刺激剂的重组牛痘病毒库存的制备。我们继续使用Mabtech Elispot系统以及U-Cytech系统，具体取决于特定的项目和合作者。在很大程度上，我们继续使用Zellnet咨询服务来记录和分析Elispot的结果。细胞免疫学核心还为研究人员提供MHC分型服务。今年已经实施了自动收费系统。今年，又有7个MHC等位基因被添加到MHC分型列表中。 在这一年里，TNPRC分配给不同项目的385只恒河猴接受了MHC-I和II基因的检测。 核心继续向调查人员提供恒河猴MHC I类四聚体。我们还建立了非放射性增殖和CTL检测。检测总数包括：A*01=96；A*02=108；A*08=97；A*11=81；B*01=97；B*03=97；B*04=97；B*08=122；B*17=122；DRB*w201=97；总计1014次。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Cellular Immunology Core provides immunology services to specific research projects of in-house and outside investigators as requested. Current services include sample preparation under standardized procedures for optimal assay analysis, planning and performance of ELISPOT assays, data processing and presentation, intracellular cytokine staining, and preparation of recombinant vaccinia viral stocks for use as antigenic stimulants in these assays. We continue to use the MabTech ELISPOT system as well as the U-Cytech system depending on the particular project and collaborators. For the most part we have continued to use the Zellnet Consulting service for recording and analyzing the ELISPOT results. The Cellular Immunology Core also provides MHC typing service to investigators. An automated charging system has been implemented this year. This year, seven more MHC alleles have been added to the MHC typing list. During this year, 385 Rhesus monkeys assigned to different projects at the TNPRC were tested for the presence of Rhesus monkey MHC-I and II genes. The core continues to provided Rhesus MHC class I tetramers to investigators. We have also established nonradioactive proliferation and CTL assays. Total numbers of assays include: A*01 = 96; A*02 = 108; A*08 = 97; A*11 = 81; B*01 = 97; B*03 = 97; B*04 = 97; B*08 = 122; B*17 = 122; DRB*w201 = 97; for a grand total of 1,014 assays.